Campbell Ian C, Sherwood Joseph M, Overby Darryl R, Hannon Bailey G, Read A Thomas, Raykin Julia, Ethier C Ross
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Rehabilitation Research and Development, Atlanta VA Medical Center, Decatur, GA, USA.
Methods Mol Biol. 2018;1695:135-159. doi: 10.1007/978-1-4939-7407-8_13.
The stiffness of the sclera is important in several ocular disorders, and there is hence a need to quantify the biomechanical properties of this tissue. Here, we present two methods for measuring the stiffness of scleral ocular tissues: ocular compliance testing and digital image correlation strain mapping. In tandem with these approaches, we provide two methods to spatially quantify the anisotropic alignment of collagen fibers making up the sclera, using second harmonic generation microscopy and small-angle light scattering. Together, these approaches allow specimen-specific measurement of tissue stiffness and collagen alignment, which are key factors in determining how the eye responds to mechanical loads.
巩膜的硬度在多种眼部疾病中都很重要，因此有必要对该组织的生物力学特性进行量化。在此，我们介绍两种测量巩膜眼部组织硬度的方法：眼顺应性测试和数字图像相关应变映射。与这些方法相结合，我们提供了两种方法，利用二次谐波产生显微镜和小角度光散射在空间上量化构成巩膜的胶原纤维的各向异性排列。这些方法共同实现了对组织硬度和胶原排列的标本特异性测量，而这两个因素是决定眼睛如何对机械负荷作出反应的关键因素。
