用于检测青光眼轴突病变和运输缺陷的顺行束路示踪

Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma.

作者信息

Crish Samuel D, Schofield Brett R

机构信息

Department of Pharmaceutical Sciences, Northeast Ohio Medical University, RGE-135, 4209 St. Rt. 44, Rootstown, OH, 44272, USA.

Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, OH, 44272, USA.

出版信息

Methods Mol Biol. 2018;1695:171-185. doi: 10.1007/978-1-4939-7407-8_15.

DOI:10.1007/978-1-4939-7407-8_15

PMID:29190027

Abstract

Whether to stage degeneration or investigate early pathology in glaucoma, examination of axonal structure and function is essential. There are a wide variety of methods available to investigators using animal models of glaucoma, with varying utilities depending on the questions asked. Here, we describe the use of anterograde neuronal tract tracing using cholera toxin B (CTB) for the determination of axon transport integrity of the retinofugal projection. This method reveals the structure of the retinal axons as well as the functional integrity of anterograde transport systems.

摘要

无论是对青光眼进行分期退变评估还是早期病理研究，轴突结构和功能的检查都至关重要。研究人员利用青光眼动物模型有多种可用方法，其效用因所提问题而异。在此，我们描述使用霍乱毒素B（CTB）进行顺行性神经元示踪以确定视网膜神经纤维投射的轴突运输完整性。该方法揭示了视网膜轴突的结构以及顺行运输系统的功能完整性。