老年大鼠大脑中的溶酶体不稳定性

Lysosome instability in aged rat brain.

作者信息

Nakamura Y, Takeda M, Suzuki H, Morita H, Tada K, Hariguchi S, Nishimura T

机构信息

Department of Neuropsychiatry, Osaka University Medical School, Japan.

出版信息

Neurosci Lett. 1989 Feb 13;97(1-2):215-20. doi: 10.1016/0304-3940(89)90166-3.

DOI:10.1016/0304-3940(89)90166-3

PMID:2919004

Abstract

The study of the age-dependent change in lysosomal enzyme activities of the cerebral tissue showed the significant increase of cathepsin D in the aged rat brain, while those of beta-glucuronidase and acid phosphatase remained unchanged. The subcellular distribution study of cathepsin D and beta-glucuronidase revealed the increased activity of these enzymes in the cytosolic fraction from the aged brain. In vitro incubation of the lysosome fraction from the aged rat brain resulted in more leakage of these two enzymes, indicating the instability of the lysosome in the aged brain, which resembled the effect of L-Leu-methyl ester to the lysosome.

摘要

对脑组织溶酶体酶活性随年龄变化的研究表明，老年大鼠脑中组织蛋白酶D显著增加，而β-葡萄糖醛酸酶和酸性磷酸酶的活性保持不变。组织蛋白酶D和β-葡萄糖醛酸酶的亚细胞分布研究显示，老年脑胞质组分中这些酶的活性增加。对老年大鼠脑溶酶体组分进行体外孵育导致这两种酶更多地泄漏，表明老年脑溶酶体不稳定，这类似于L-亮氨酸甲酯对溶酶体的作用。