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使用两种荧光染料通过流式细胞术鉴别七种谱系标志物。

Flow cytometric discrimination of seven lineage markers by using two fluorochromes.

作者信息

Boin Francesco, Giardino Torchia Maria Letizia, Borrello Ivan, Noonan Kimberly A, Neil Matthew, Soloski Mark J, Cimbro Raffaello

机构信息

Division of Rheumatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.

Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America.

出版信息

PLoS One. 2017 Nov 30;12(11):e0188916. doi: 10.1371/journal.pone.0188916. eCollection 2017.

DOI:10.1371/journal.pone.0188916
PMID:29190813
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5708788/
Abstract

Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.

摘要

流式细胞术是用于分析复杂细胞群体多种参数的主要免疫学技术。我们提出了一种染色方法，仅使用两种荧光染料和最少数量的细胞就能识别主要的人类单核淋巴细胞和髓细胞群体（CD4+和CD8+ T细胞、γδ T细胞、B细胞、NK细胞和单核细胞）。我们的方法增加了大多数流式细胞仪上可记录的标志物数量，从而实现更深入、更全面的免疫表型分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4f9/5708788/35ee5e1deaa4/pone.0188916.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4f9/5708788/da28c3b0e56d/pone.0188916.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4f9/5708788/35ee5e1deaa4/pone.0188916.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4f9/5708788/da28c3b0e56d/pone.0188916.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4f9/5708788/35ee5e1deaa4/pone.0188916.g002.jpg

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