Novikoff A B, Novikoff P M, Stockert R J, Becker F F, Yam A, Poruchynsky M S, Levin W, Thomas P E
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5207-11. doi: 10.1073/pnas.76.10.5207.
A knowledge of the biological characteristics of carcinogen-induced hyperplastic nodules of rat liver may be important in the understanding of cancer development. Although its biological role remains to be elucidated, the level of microsomal epoxide hydrase (epoxide hydrolase, EC 3.3.2.3) is 5- to 7-fold greater in hyperplastic nodules nodules induced by feeding the hepatocarcinogen 2-acetylaminofluorene than in liver of control rats. After removal of the carcinogen from the diet, the high level of the enzyme is maintained in those nodules that persist and in the hepatocellular carcinomas that subsequently develop. The availability of antibody to the epoxide hydrase made it possible to use electron microscopic immunocytochemistry to localize this enzyme in the cells of hyperplastic nodules. The immunocytochemical procedure provides direct visual evidence for the presence of this enzyme in smooth endoplasmic reticulum and also in rough endoplasmic reticulum (including the nuclear envelope) of the nodule's parenchymal cells.
了解致癌物诱导的大鼠肝脏增生性结节的生物学特性，对于理解癌症发展可能具有重要意义。尽管其生物学作用尚待阐明，但通过喂食肝癌致癌物2-乙酰氨基芴诱导的增生性结节中，微粒体环氧化物水解酶（环氧化物水解酶，EC 3.3.2.3）的水平比对照大鼠肝脏中的高5至7倍。从饮食中去除致癌物后，该酶的高水平在持续存在的结节以及随后发展的肝细胞癌中得以维持。环氧化物水解酶抗体的可得性使得利用电子显微镜免疫细胞化学将该酶定位在增生性结节的细胞中成为可能。免疫细胞化学方法为该酶存在于结节实质细胞的滑面内质网以及粗面内质网（包括核膜）中提供了直接的视觉证据。
