Vecchi M, Sakamaki S, Diamond B, Novikoff A B, Novikoff P M, Das K M
Proc Natl Acad Sci U S A. 1987 May;84(10):3425-9. doi: 10.1073/pnas.84.10.3425.
A mouse monoclonal antibody (7E6A5) of IgG isotype, reacting specifically with mucin-producing goblet cells of the human gastrointestinal tract, has been developed. 7E6A5 reacts by an ELISA with colonic protein eluted from a DEAE column. A screening by immunoperoxidase assay of 76 specimens from 19 different human tissues showed that the immunoreactivity of 7E6A5 was confined exclusively in the globules of goblet cells in the colon, the appendix, and the small intestine. Nongoblet small and large intestinal epithelial cells did not react. Immunoelectron microscopy demonstrated the reactivity with mucin droplets in a homogeneous granular pattern inside the globules of goblet cells. Mucus-secreting cells from remaining parts of the gastrointestinal tract and other mucus-secreting organs such as respiratory, genitourinary tracts, salivary and mammary glands did not show any reactivity to 7E6A5. These findings indicate that the antigen recognized by 7E6A5 is shared by the goblet cells of both the small and large intestines and is unique to them. The monoclonal antibody may be useful in the study of function of mucus-secreting goblet cells and may represent an important tool in the evaluation of diseases such as ulcerative colitis, colon cancer, and intestinal metaplasia in gastric mucosa that are associated with quantitative changes in goblet cell numbers or with qualitative differences in mucin secretion.
已研制出一种IgG同型的小鼠单克隆抗体（7E6A5），它能与人胃肠道中产生粘蛋白的杯状细胞特异性反应。7E6A5通过酶联免疫吸附测定法与从二乙氨基乙基（DEAE）柱洗脱的结肠蛋白发生反应。通过免疫过氧化物酶测定法对来自19种不同人体组织的76个标本进行筛选，结果显示7E6A5的免疫反应性仅局限于结肠、阑尾和小肠中杯状细胞的小球内。非杯状的小肠和大肠上皮细胞无反应。免疫电子显微镜显示，7E6A5与杯状细胞小球内呈均匀颗粒状的粘蛋白滴发生反应。胃肠道其他部位以及呼吸道、泌尿生殖道、唾液腺和乳腺等其他分泌粘液的器官中的粘液分泌细胞对7E6A5均无反应。这些发现表明，7E6A5识别的抗原为小肠和大肠的杯状细胞所共有，且仅存在于这些细胞中。该单克隆抗体可能有助于研究分泌粘液的杯状细胞的功能，并且可能是评估诸如溃疡性结肠炎、结肠癌以及胃粘膜肠化生等与杯状细胞数量的定量变化或粘蛋白分泌的定性差异相关疾病的重要工具。
