Waechter F, Bentley P, Germann M, Oesch F, Stäubli W
Biochem J. 1982 Mar 15;202(3):677-86. doi: 10.1042/bj2020677.
The distribution of rat liver epoxide hydrolase in various subcellular fractions was investigated by immuno-electron-microscopy. Ferritin-linked monospecific anti-(epoxide hydrolase) immunoglobulins bound specifically to the cytoplasmic surfaces of total microsomal preparations and smooth and rough microsomal fractions as well as the nuclear envelope. Specific binding was not observed when the ferritin conjugates were incubated with peroxisomes, lysosomes and mitochondria. The average specific ferritin load of the individual subcellular fractions correlated well with the measured epoxide hydrolase activities. This correlation was observed with fractions prepared from control, phenobarbitone-treated and 2-acetamidofluorene-treated rats.
通过免疫电子显微镜研究了大鼠肝脏环氧化物水解酶在各种亚细胞组分中的分布。铁蛋白连接的单特异性抗（环氧化物水解酶）免疫球蛋白特异性结合到总微粒体制剂、光滑和粗糙微粒体组分以及核膜的细胞质表面。当铁蛋白缀合物与过氧化物酶体、溶酶体和线粒体一起孵育时，未观察到特异性结合。各个亚细胞组分的平均特异性铁蛋白负载量与测得的环氧化物水解酶活性密切相关。在从对照、苯巴比妥处理和2-乙酰氨基芴处理的大鼠制备的组分中观察到了这种相关性。
