Institute for Microbiology,, University of Greifswald, Greifswald, Germany.
Institute of Marine Biotechnology, Greifswald, Germany.
Proteomics. 2018 Jan;18(1). doi: 10.1002/pmic.201700109.
Since starvation for carbon sources is a common condition for bacteria in nature and it can also occur in industrial fermentation processes due to mixing zones, knowledge about the response of cells to carbon starvation is beneficial. The preferred carbon source for bacilli is glucose. The response of Bacillus pumilus cells to glucose starvation using metabolic labeling and quantitative proteomics was analyzed. Glucose starvation led to an extensive reprogramming of the protein expression pattern in B. pumilus. The amounts of proteins of the central carbon metabolic pathways (glycolysis and TCC) remained stable in starving cells. Proteins for gluconeogenesis were found in higher amounts during starvation. Furthermore, many proteins involved in acquisition and usage of alternative carbon sources were present in elevated amounts in starving cells. Enzymes for fatty acid degradation and proteases and peptidases were also found in higher abundance when cells entered stationary phase. Among the proteins found in lower amounts were many enzymes involved in amino acid and nucleotide synthesis and several NRPS and PKS proteins.
由于碳源饥饿是自然界中细菌的常见状态，并且由于混合区，它也会在工业发酵过程中发生，因此了解细胞对碳饥饿的反应是有益的。芽孢杆菌的首选碳源是葡萄糖。使用代谢标记和定量蛋白质组学分析了枯草芽孢杆菌细胞对葡萄糖饥饿的反应。葡萄糖饥饿导致枯草芽孢杆菌的蛋白质表达模式发生广泛重编程。在饥饿细胞中，中心碳代谢途径（糖酵解和 TCC）的蛋白质含量保持稳定。在饥饿期间发现了用于糖异生的蛋白质的含量更高。此外，许多参与获取和使用替代碳源的蛋白质在饥饿细胞中以更高的含量存在。脂肪酸降解酶以及蛋白酶和肽酶在细胞进入静止期时也以更高的丰度存在。在含量较低的蛋白质中，有许多参与氨基酸和核苷酸合成的酶，以及几种 NRPS 和 PKS 蛋白。
