Igeh Patience C, Dos Santos Quinton M, Avenant-Oldewage Annemariè
Department of Zoology, University of Johannesburg, PO Box 524, Auckland Park 2006, Johannesburg, South Africa - Department of Microbiology, Plateau State University, PO Box 2012, Jos, Nigeria.
Department of Zoology, University of Johannesburg, PO Box 524, Auckland Park 2006, Johannesburg, South Africa.
Parasite. 2017;24:49. doi: 10.1051/parasite/2017046. Epub 2017 Dec 1.
The sclerotized structures of monogeneans have traditionally been studied by light microscopy and different staining techniques. Recently, enzymatic digestion followed by scanning electron microscopy (SEM) has enabled the examination of structural details not visible with light microscopy. In order to obtain better, and more accurate, morphological information on sclerotized structures not affected by mounting medium or cover slip pressure, the sclerites of Cichlidogyrus philander Douëllou, 1993 (Monogenea, Ancyrocephalidae), collected from Pseudocrenilabrus philander (Weber, 1897), were redescribed using SEM. Parasites were collected from Padda Dam, Gauteng, South Africa and soft tissue was digested to release the sclerotized structures. The digested tissue also provided sufficient genetic material for molecular characterization of this species. Cichlidogyrus philander is characterised by a penis with a sharp, curved, lateral termination, an accessory piece with a hook-like extremity that may appear forked terminally, and lack of a visible vagina. The transverse bars have concave and convex surfaces with ribs on the concave surface. The dorsal bar bears fenestrations at the base of the auricles and the ventral and dorsal gripi are dissimilar. Furthermore, the large first pair of uncinuli shows lateral wings on the left side of the base. On top of this wing, a ball-like structure with a small fenestration is visible. Genetic characters derived from the 28S rDNA, the COI mitochondrial DNA and ITS1 rDNA regions distinguish C. philander from all other Cichlidogyrus sequenced species.
单殖吸虫的硬化结构传统上是通过光学显微镜和不同的染色技术进行研究的。最近，酶消化后再进行扫描电子显微镜（SEM）检查，能够观察到光学显微镜下不可见的结构细节。为了获得关于不受封固介质或盖玻片压力影响的硬化结构的更好、更准确的形态学信息，我们使用SEM对1993年Douëllou发现的菲氏丽体盘虫（Cichlidogyrus philander）（单殖吸虫纲，锚首虫科）的硬棘进行了重新描述，该虫采自秀丽非鲫（Pseudocrenilabrus philander）（Weber，1897）。寄生虫采自南非豪登省的帕达大坝，消化软组织以释放硬化结构。消化后的组织还为该物种的分子特征提供了足够的遗传物质。菲氏丽体盘虫的特征是阴茎有一个尖锐、弯曲的侧向末端，一个附属片有一个钩状末端，末端可能呈叉状，且没有可见的阴道。横杆有凹面和凸面，凹面上有肋条。背杆在耳廓基部有小孔，腹侧和背侧握钩不同。此外，第一对大钩在基部左侧有侧翼。在这个侧翼顶部，可以看到一个有小窗孔的球状结构。来自28S rDNA、COI线粒体DNA和ITS1 rDNA区域的遗传特征将菲氏丽体盘虫与所有其他已测序的丽体盘虫属物种区分开来。
