分析卵巢子宫内膜异位症囊液中的细胞外酶 ADA、ALP、ENPP1 和 ENPP3，作为子宫内膜异位症的候选生物标志物。

Analysis of the ectoenzymes ADA, ALP, ENPP1, and ENPP3, in the contents of ovarian endometriomas as candidate biomarkers of endometriosis.

机构信息

Department de Patologia i Terapèutica Experimental, Facultat de Medicina i Ciències de la Salut, Campus Bellvitge, Universitat de Barcelona, Barcelona, Spain.

Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), Oncobell Program, CIBERONC, Barcelona, Spain.

出版信息

Am J Reprod Immunol. 2018 Feb;79(2). doi: 10.1111/aji.12794. Epub 2017 Nov 30.

DOI:10.1111/aji.12794

PMID:29194839

Abstract

PROBLEM

The diagnosis of endometriosis, a prevalent chronic disease with a strong inflammatory component, is usually delayed due to the lack of noninvasive diagnostic tests. Purinergic signaling, a key cell pathway, is altered in many inflammatory disorders. The aim of the present work was to evaluate the levels of adenosine deaminase (ADA), alkaline phosphatase (ALP), ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), and ENPP3, elements of purinergic signaling, as biomarker candidates for endometriosis.

METHOD OF STUDY

A case-control comparative study was conducted to determine ADA, ALP, ENPP1 and ENPP3 levels in echo-guided aspirated fluids of endometriomas (case group) and simple ovarian cysts (control group) using the ELISA technique.

RESULTS

Adenosine deaminase, ALP, ENPP1, and ENPP3 were present and quantifiable in the contents of endometriomas and simple cysts. There were significant differences in ADA and ENPP1 levels in endometriomas in comparison with simple cysts (2787 U/L and 103.9 ng/mL more in endometriomas, for ADA and ENPP1, respectively). Comparisons of ALP and ENPP3 levels between the two groups did not reveal significant differences.

CONCLUSION

The ectoenzymes ADA and ENPP1 are biomarker candidates for endometriosis.

摘要

问题

子宫内膜异位症是一种常见的慢性疾病，具有强烈的炎症成分，由于缺乏非侵入性诊断测试，其诊断通常会被延误。嘌呤能信号转导是一种关键的细胞途径，在许多炎症性疾病中都会发生改变。本研究旨在评估腺苷脱氨酶（ADA）、碱性磷酸酶（ALP）、外核苷酸焦磷酸酶/磷酸二酯酶 1（ENPP1）和 ENPP3 等嘌呤能信号转导元件的水平，作为子宫内膜异位症的生物标志物候选物。

研究方法

采用病例对照比较研究，使用 ELISA 技术测定回声引导抽吸的子宫内膜异位症（病例组）和单纯卵巢囊肿（对照组）囊液中的 ADA、ALP、ENPP1 和 ENPP3 水平。

结果

ADA、ALP、ENPP1 和 ENPP3 在子宫内膜异位症和单纯囊肿的囊液中均存在且可定量。与单纯囊肿相比，子宫内膜异位症中的 ADA 和 ENPP1 水平存在显著差异（ADA 和 ENPP1 分别高出 2787 U/L 和 103.9ng/mL）。两组间 ALP 和 ENPP3 水平的比较未显示出显著差异。