Nauroze Tooba, Ali Shaukat, Kanwal Lubna, Ara Chaman, Akbar Mughal Tufail, Andleeb Shagufta
Applied Entomology and Medical Toxicology Laboratory, Department of Zoology, Government College University, Lahore, Pakistan.
Department of Zoology, University of Education, Lahore, Pakistan.
Saudi J Biol Sci. 2023 Mar;30(3):103571. doi: 10.1016/j.sjbs.2023.103571. Epub 2023 Jan 25.
Hexavalent chromium induces oxidative stress in the liver and kidney. Therefore an study was designed to investigate the modulatory effect of biosynthesized AgNP against Cr (VI) induced hepatotoxicity and nephrotoxicity. The organs index, serum level of ALT, AST, ALP, MDA, total protein and creatinine were measured. The histopathology and micrometry of the liver and kidney were examined. The liver index was significantly increased (0.098 ± 0.13 g) with slight increase in kidney index in Cr exposed group. The serum level of ALT (163.0 ± 5.5 U/L), AST (484.0 ± 10.7 U/L), ALP (337.6 ± 9.6 U/L), MDA (641.2 ± 29.2 U/L), and creatinine (2.9 ± 0.2 mg/dL) were significantly increased ( ≤ 0.05) with significant decrease in total protein level (2.9 ± 0.2 g/dL) ( ≤ 0.05) in chromium treated group. In histopathology, distorted hepatic cords, necrosis, damaged glomerulus and Bowman's capsule were observed. Micrometric studies of the liver and kidney showed significant increase in size of hepatocytes (1188.2 ± 467.7 µ) and their nuclei (456.4 ± 206.7 µ), ACSA of Bowman's capsule (11835.5 ± 336.7 µ) and glomerulus (9051.8 ± 249.8 µ in Cr (VI) treated group. The size of brush border (10.1 ± 3.0 µ) was significantly reduced in Cr (VI) treated group however the ACSA of lumen was not significantly changed. With the administration of NSSE and AgNPs, decreased the oxidative damage caused by Cr (V).
六价铬会在肝脏和肾脏中引发氧化应激。因此，设计了一项研究来探究生物合成的银纳米颗粒对六价铬诱导的肝毒性和肾毒性的调节作用。测量了器官指数、血清中的谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）、丙二醛（MDA）、总蛋白和肌酐水平。对肝脏和肾脏进行了组织病理学和显微镜测量检查。在铬暴露组中，肝脏指数显著增加（0.098±0.13克），肾脏指数略有增加。在铬处理组中，血清中的谷丙转氨酶（163.0±5.5 U/L）、谷草转氨酶（484.0±10.7 U/L）、碱性磷酸酶（337.6±9.6 U/L）、丙二醛（641.2±29.2 U/L）和肌酐（2.9±0.2 mg/dL）显著增加（P≤0.05），而总蛋白水平显著降低（2.9±0.2 g/dL）（P≤0.05）。在组织病理学方面，观察到肝索扭曲、坏死、肾小球和鲍曼囊受损。肝脏和肾脏的显微镜测量研究显示，在六价铬处理组中，肝细胞大小（1188.2±467.7 µm）及其细胞核大小（456.4±206.7 µm）、鲍曼囊的平均截面积（11835.5±336.7 µm）和肾小球（9051.8±249.8 µm）显著增加。在六价铬处理组中，刷状缘大小（10.1±3.0 µm）显著减小，但管腔的平均截面积没有显著变化。随着非皂化皂树提取物（NSSE）和银纳米颗粒的给药，六价铬造成的氧化损伤有所减轻。
