Wang Xu, Zhou Zhenqi, Chai Xinrui, Li Jie, Wang Wannian, Pang Zhixu, Cheng Lifen, Cheng Caihong, Qiao Liying, Pan Yangyang, Yang Kaijie, Liu Wenzhong, Liu Jianhua
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China.
Shanxi Animal Husbandry Technology Extension Service Center, Taiyuan 030001, China.
Animals (Basel). 2024 Dec 26;15(1):36. doi: 10.3390/ani15010036.
Lüliang black goat (LBG) is a unique livestock genetic resource of great significance for the local agriculture and economic development of Shanxi, China. However, the kidding rate of LBG is relatively low, which limits efforts to improve the reproductive performance and economic benefits of this breed. Therefore, improving the kidding characteristics of LBG is crucial for increasing its economic benefits. In this study, 20 LBG does were selected for whole-genome resequencing and divided into two groups: 10 in the T group (does with three consecutive kiddings of twin kids) and 10 in the S group (does with three consecutive kiddings of single kids). Based on whole-genome resequencing data, this study comprehensively assessed the population structure and genetic diversity of LBG and explored the related genes that affect reproductive performance. Three selection signal analysis methods-Fst (Fixation Index), π (nucleotide diversity), and XP-CLR (Cross Population Composite Likelihood Ratio)-were applied to screen a total of 838 genes, and enrichment analysis was performed to identify genes closely related to the reproductive performance of LBG, including , , and . A generalized linear model was used to conduct a correlation analysis between non-synonymous mutations in the three genes and the number of kids produced. Two loci that were significantly correlated with kidding number were identified ( < 0.05): g.63400363 C>T and g.63417538 C>T. In general, the LBG population has high genetic diversity and good prospects for genetic improvement. The findings revealed that mining high-fecundity selection characteristics provides a basis for research on goat reproductive mechanisms.
吕梁黑山羊（LBG）是一种独特的家畜遗传资源，对中国山西省当地农业和经济发展具有重要意义。然而，吕梁黑山羊的产羔率相对较低，这限制了提高该品种繁殖性能和经济效益的努力。因此，改善吕梁黑山羊的产羔特性对提高其经济效益至关重要。在本研究中，选取了20只吕梁黑山羊母羊进行全基因组重测序，并分为两组：T组（连续三胎产双羔的母羊）10只和S组（连续三胎产单羔的母羊）10只。基于全基因组重测序数据，本研究全面评估了吕梁黑山羊的群体结构和遗传多样性，并探索了影响繁殖性能的相关基因。应用三种选择信号分析方法——Fst（固定指数）、π（核苷酸多样性）和XP-CLR（跨群体复合似然比）——共筛选出838个基因，并进行富集分析以鉴定与吕梁黑山羊繁殖性能密切相关的基因，包括[具体基因未给出]。使用广义线性模型对这三个基因中的非同义突变与产羔数之间进行相关性分析。确定了两个与产羔数显著相关的位点（P<0.05）：g.63400363 C>T和g.63417538 C>T。总体而言，吕梁黑山羊群体具有较高的遗传多样性和良好的遗传改良前景。研究结果表明，挖掘高繁殖力选择特征为山羊繁殖机制的研究提供了依据。
