正常人骨髓培养中尿卟啉原I合酶的诱导：红系分化的早期定量反应。
Uroporphyrinogen I synthase induction in normal human bone marrow cultures: an early and quantitative response of erythroid differentiation.
作者信息
Sassa S, Urabe A
出版信息
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5321-5. doi: 10.1073/pnas.76.10.5321.
Abstract
Uroporphyrinogen I (URO) synthase [porphobilinogen ammonia-lyase (polymerizing), EC 4.3.1.8] activity increased when cultures of normal human bone marrow cells were incubated with erythropoietin. The increase of URO synthase activity was a linear function of erythropoietin concentration in the culture medium and was proportional to the extent of heme synthesis as determined by 55Fe incorporation into heme. The onset of the increase in URO synthase activity, which occurred on day 4 of incubation with erythropoietin, preceded by 3 days the appearance of hemoglobin in colonies derived from erythroid progenitor cells. These results indicate that induction of URO synthase in normal human bone marrow cells incubated with erythropoietin is an early event and that URO synthase activity is a useful quantitative index of erythroid differentiation.
摘要
当正常人骨髓细胞培养物与促红细胞生成素一起孵育时，尿卟啉原I（URO）合酶[胆色素原氨裂解酶（聚合），EC 4.3.1.8]的活性增加。URO合酶活性的增加是培养基中促红细胞生成素浓度的线性函数，并且与通过55Fe掺入血红素所确定的血红素合成程度成比例。URO合酶活性增加的起始发生在与促红细胞生成素孵育的第4天，比红系祖细胞衍生的集落中血红蛋白的出现提前3天。这些结果表明，与促红细胞生成素一起孵育的正常人骨髓细胞中URO合酶的诱导是一个早期事件，并且URO合酶活性是红系分化的一个有用的定量指标。
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Uroporphyrinogen I synthase induction in normal human bone marrow cultures: an early and quantitative response of erythroid differentiation.正常人骨髓培养中尿卟啉原I合酶的诱导：红系分化的早期定量反应。
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5321-5. doi: 10.1073/pnas.76.10.5321.
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本文引用的文献
1
The activities of three enzymes of haem synthesis during hepatic erythropoiesis in the mouse embryo.
J Embryol Exp Morphol. 1971 Oct;26(2):313-22.
2
Erythroid colony formation in cultures of mouse and human bone marrow: analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose-concanavalin A.小鼠和人骨髓培养中的红系集落形成：通过琼脂糖-伴刀豆球蛋白A凝胶过滤和亲和层析分析对促红细胞生成素的需求
J Cell Physiol. 1974 Apr;83(2):309-20. doi: 10.1002/jcp.1040830218.
3
Studies in lead poisoning. I. Microanalysis of erythrocyte protoporphyrin levels by spectrophotometry in the detection of chronic lead intoxication in the subclinical range.铅中毒研究。I. 用分光光度法对红细胞原卟啉水平进行微量分析以检测亚临床范围内的慢性铅中毒。
Biochem Med. 1973 Aug;8(1):135-48. doi: 10.1016/0006-2944(73)90017-3.
4
A microassay for uroporphyrinogen I synthase, one of three abnormal enzyme activities in acute intermittent porphyria, and its application to the study of the genetics of this disease.急性间歇性卟啉病三种异常酶活性之一的尿卟啉原I合酶的微量测定法及其在该疾病遗传学研究中的应用。
Proc Natl Acad Sci U S A. 1974 Mar;71(3):732-6. doi: 10.1073/pnas.71.3.732.
5
Erythropietic colonies in cultures of human marrow.
Blood. 1974 Nov;44(5):659-69.
6
Linear sequential arrangement of genes for the biosynthetic pathway of protoheme in Staphylococcus aureus.金黄色葡萄球菌中原卟啉生物合成途径相关基因的线性序列排列
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7
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Cell. 1976 Aug;8(4):513-20. doi: 10.1016/0092-8674(76)90219-1.
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