正常人骨髓培养中尿卟啉原I合酶的诱导：红系分化的早期定量反应。
Uroporphyrinogen I synthase induction in normal human bone marrow cultures: an early and quantitative response of erythroid differentiation.
作者信息
Sassa S, Urabe A
出版信息
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5321-5. doi: 10.1073/pnas.76.10.5321.
Abstract
Uroporphyrinogen I (URO) synthase [porphobilinogen ammonia-lyase (polymerizing), EC 4.3.1.8] activity increased when cultures of normal human bone marrow cells were incubated with erythropoietin. The increase of URO synthase activity was a linear function of erythropoietin concentration in the culture medium and was proportional to the extent of heme synthesis as determined by 55Fe incorporation into heme. The onset of the increase in URO synthase activity, which occurred on day 4 of incubation with erythropoietin, preceded by 3 days the appearance of hemoglobin in colonies derived from erythroid progenitor cells. These results indicate that induction of URO synthase in normal human bone marrow cells incubated with erythropoietin is an early event and that URO synthase activity is a useful quantitative index of erythroid differentiation.
摘要
当正常人骨髓细胞培养物与促红细胞生成素一起孵育时，尿卟啉原I（URO）合酶[胆色素原氨裂解酶（聚合），EC 4.3.1.8]的活性增加。URO合酶活性的增加是培养基中促红细胞生成素浓度的线性函数，并且与通过55Fe掺入血红素所确定的血红素合成程度成比例。URO合酶活性增加的起始发生在与促红细胞生成素孵育的第4天，比红系祖细胞衍生的集落中血红蛋白的出现提前3天。这些结果表明，与促红细胞生成素一起孵育的正常人骨髓细胞中URO合酶的诱导是一个早期事件，并且URO合酶活性是红系分化的一个有用的定量指标。
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