Chen Zhonglin, Zhou Qian, Guan Wei, Wang Jie, Li Yue, Yu Ning, Wei Jie
School of Environmental Science, Liaoning University, Shenyang 110036, PR China.
School of Environmental Science, Liaoning University, Shenyang 110036, PR China; College of Chemistry, Liaoning University, Shenyang 110036, PR China.
Chemosphere. 2018 Mar;194:20-27. doi: 10.1016/j.chemosphere.2017.11.145. Epub 2017 Nov 24.
The effects of five imidazolium ionic liquids with different anions were studied in hydroponically grown wheat seedlings at concentrations of 50, 100, 150, 200, 250, and 300 mg L. The results showed that shoots and roots grew shorter and dry weight decreased with increasing concentrations of ionic liquids. Moreover, the antioxidant enzyme activities decreased and malondialdehyde (MDA) content was greater in the leaves of wheat seedlings subjected to ionic liquid (IL) treatments. The order of influence of ionic liquids on these indexes was [Cmim][TfO]> [Cmim][Cl]> [Cmim][BF]> [Cmim][Lact]> [Cmim][Ala]. A transmission electron microscope (TEM) was used to observe leaf and root cellular structures, such as chloroplast, nucleus, mitochondria, and rough endoplasmic reticulum, in wheat exposed to ionic liquids at a concentration of 150 mg L. The results showed that the cellular structures of wheat were affected, and the degree of the effect of five ILs was consistent with the general trend of the measured indexes in this study. Ionic liquids influence the growth of plants by impeding growth, disrupting metabolic physiology and changing cellular structures. The degree of toxicity of imidazolium-based ionic liquids with different anions varies.
研究了5种不同阴离子的咪唑鎓离子液体对水培小麦幼苗的影响，浓度分别为50、100、150、200、250和300 mg/L。结果表明，随着离子液体浓度的增加，地上部和根部生长变矮，干重降低。此外，经离子液体（IL）处理的小麦幼苗叶片中抗氧化酶活性降低，丙二醛（MDA）含量增加。离子液体对这些指标的影响顺序为[Cmim][TfO]>[Cmim][Cl]>[Cmim][BF]>[Cmim][Lact]>[Cmim][Ala]。利用透射电子显微镜（TEM）观察了浓度为150 mg/L离子液体处理下小麦叶片和根部的细胞结构，如叶绿体、细胞核、线粒体和粗面内质网。结果表明，小麦的细胞结构受到影响，5种离子液体的影响程度与本研究中测定指标的总体趋势一致。离子液体通过阻碍生长、扰乱代谢生理和改变细胞结构来影响植物生长。不同阴离子的咪唑基离子液体的毒性程度各不相同。
