微小RNA-875-5p抑制SATB2以促进肺癌细胞的侵袭。

The miR-875-5p inhibits SATB2 to promote the invasion of lung cancer cells.

作者信息

Wang Jun, Lu Yadong, Ding Hao, Gu Tao, Gong Chenhu, Sun Jianfei, Zhang Zhihong, Zhao Yucai, Ma Chunping

机构信息

Zhangjiagang First People 's Hospital, Zhangjiagang Hospital Affiliated to Suzhou University, Zhangjiagang 215600, China.

出版信息

Gene. 2018 Feb 20;644:13-19. doi: 10.1016/j.gene.2017.11.066. Epub 2017 Nov 28.

DOI:10.1016/j.gene.2017.11.066

PMID:29196257

Abstract

The pathogenesis of non-small cell lung cancer (NSCLC) is regulated by various miRNAs. In this study, we identified that miR-875-5pis up-regulated in NSCLC patients, and inhibited SATB Homeobox 2(SATB2) to promote proliferation and invasion of NSCLCcells.CCK-8assay revealed thatmiR-875-5p mimics promoted proliferation of NSCLC cells. Transwell assay showed that miR-875-5pmimicspromoted the invasion and migration of NSCLC cells. Luciferase assays confirmed that miR-875-5pdirectly binds to the 3'untranslated region of SATB2, and western blotting showed that miR-875-5psuppresses the expression of SATB2 at the protein level. Moreover, the inhibitors of miR-875-5pinhibit proliferation and invasion of NSCLC cell lines. The miR-875-5pwouldbe a potential therapeutic target for NSCLC treatment in the future.

摘要

非小细胞肺癌（NSCLC）的发病机制受多种微小RNA（miRNA）调控。在本研究中，我们发现miR-875-5p在NSCLC患者中上调，并通过抑制SATB同源盒2（SATB2）来促进NSCLC细胞的增殖和侵袭。CCK-8检测显示，miR-875-5p模拟物促进NSCLC细胞增殖。Transwell检测表明，miR-875-5p模拟物促进NSCLC细胞的侵袭和迁移。荧光素酶检测证实miR-875-5p直接与SATB2的3'非翻译区结合，蛋白质印迹法显示miR-875-5p在蛋白质水平上抑制SATB2的表达。此外，miR-875-5p抑制剂可抑制NSCLC细胞系的增殖和侵袭。miR-875-5p将来可能成为NSCLC治疗的潜在靶点。