Xie Xiao, Liu Hong-Tao, Mei Ju, Ding Fang-Bao, Xiao Hai-Bo, Hu Feng-Qing, Hu Rui, Wang Ming-Song
Department of Cardio-Thoracic Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092, P. R. China.
Department of Cardio-Thoracic Surgery, The first Affiliated Hospital of Soochow University Suzhou 215006, P. R. China.
Int J Clin Exp Pathol. 2015 Apr 1;8(4):3827-34. eCollection 2015.
MicroRNAs are a class of small non-coding RNAs that play essential roles in cancer development and progression. Recent studies suggested that abnormal expression of miRNAs occurs frequently in non-small cell lung cancer (NSCLC) tissues compared to adjacent normal tissues. In this study, we investigated the expression and the biological roles of miR-106a in non-small cell lung cancer. Our results showed that miR-106a was up-regulated in NSCLC tissues and cell lines. Inhibition of miR-106a in NSCLC cells substantially inhibited cell proliferation, migration, and invasion. Phosphatase and tensin homolog (PTEN) was identified as a direct target of miR-106a, and over-expression of miR-106a suppressed PTEN by direct binding to its 3'-untranslated region (3'-UTR). Furthermore, the presence of miR-106a was inversely correlated with PTEN in NSCLC tissues. Overall, this study suggested that miR-106a inhibited the growth and metastasis of NSCLC cells by decreasing PTEN expression. These data provide novel insights with potential therapeutic applications for the treatment of NSCLC.
微小RNA是一类小的非编码RNA，在癌症的发生和发展中起着至关重要的作用。最近的研究表明，与相邻正常组织相比，微小RNA在非小细胞肺癌（NSCLC）组织中经常出现异常表达。在本研究中，我们调查了miR-106a在非小细胞肺癌中的表达及其生物学作用。我们的结果表明，miR-106a在NSCLC组织和细胞系中上调。抑制NSCLC细胞中的miR-106a可显著抑制细胞增殖、迁移和侵袭。磷酸酶和张力蛋白同源物（PTEN）被确定为miR-106a的直接靶点，miR-106a的过表达通过直接结合其3'非翻译区（3'-UTR）抑制PTEN。此外，在NSCLC组织中，miR-106a的存在与PTEN呈负相关。总体而言，本研究表明miR-106a通过降低PTEN表达来抑制NSCLC细胞的生长和转移。这些数据为NSCLC的治疗提供了具有潜在治疗应用的新见解。
