Huang Kui, Peng Lu, Liu Yingying, Yao Rundong, Liu Zhibin, Li Xufeng, Yang Yi, Wang Jianmei
Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, PR China.
Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, PR China.
Biochem Biophys Res Commun. 2018 Mar 25;498(1):92-98. doi: 10.1016/j.bbrc.2017.11.175. Epub 2017 Dec 2.
The calcium-dependent protein kinases (CDPKs) play vital roles in plant response to various environmental stimuli. Here, we investigated the function of Arabidopsis AtCPK1 in response to salt and drought stress. The loss-of-function cpk1 mutant displayed hypersensitive to salt and drought stress, whereas overexpressing AtCPK1 in Arabidopsis plants significantly enhanced the resistance to salt or drought stress. The reduced or elevated tolerance of cpk1 mutant and AtCPK1-overexpressing lines was confirmed by the changes of proline, malondialdehyde (MDA) and HO. Real-time PCR analysis revealed that the expression of several stress-inducible genes (RD29A, COR15A, ZAT10, APX2) down-regulated in cpk1 mutant and up-regulated in AtCPK1-overexpressing plants. These results are likely to indicate that AtCPK1 positively regulates salt and drought stress in Arabidopsis.
钙依赖蛋白激酶（CDPKs）在植物对各种环境刺激的响应中发挥着至关重要的作用。在此，我们研究了拟南芥AtCPK1在响应盐胁迫和干旱胁迫中的功能。功能缺失的cpk1突变体对盐胁迫和干旱胁迫表现出超敏反应，而在拟南芥植株中过表达AtCPK1则显著增强了对盐胁迫或干旱胁迫的抗性。脯氨酸、丙二醛（MDA）和HO的变化证实了cpk1突变体和AtCPK1过表达株系的耐受性降低或升高。实时PCR分析表明，几个胁迫诱导基因（RD29A、COR15A、ZAT10、APX2）的表达在cpk1突变体中下调，而在AtCPK1过表达植株中上调。这些结果可能表明AtCPK1在拟南芥中正向调节盐胁迫和干旱胁迫。
