Kumar Ranjeet R, Niraj Ravi K, Goswami Suneha, Thimmegowda Vinutha, Mishra Gyan P, Mishra Dwijesh, Rai Gyanendra K, Kumar Soora Naresh, Viswanathan Chinnusamy, Tyagi Aruna, Singh Gyanendra P, Rai Anil K
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110012 India.
Division of Seed Technology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India.
3 Biotech. 2024 Jun;14(6):150. doi: 10.1007/s13205-024-03989-6. Epub 2024 May 7.
Calcium-dependent protein kinase (CDPK) is member of one of the most important signalling cascades operating inside the plant system due to its peculiar role as thermo-sensor. Here, we identified 28 full length putative from wheat designated as (1-28). Based on digital gene expression, we cloned full length TaCPK-1 gene of 1691 nucleotides with open reading frame (ORF) of 548 amino acids (accession number OP125853). The expression of was observed maximum (3.1-fold) in leaf of wheat . HD2985 (thermotolerant) under (38 ± 3 °C, 2 h), as compared to control. A positive correlation was observed between the expression of and other stress-associated genes (, , , , , , and ) involved in thermotolerance. Global protein kinase assay showed maximum activity in leaves, as compared to root, stem and spike under heat stress. Immunoblot analysis showed abundance of CDPK protein in wheat . HD2985 (thermotolerant) in response to (38 ± 3 °C, 2 h), as compared to HD2329 (thermosusceptible). Calcium ion (Ca), being inducer of CDPK, showed strong Ca-signature in the leaf tissue (Ca-622 ppm) of thermotolerant wheat . under heat stress, whereas it was minimum (Ca-201 ppm) in spike tissue. We observed significant variations in the ionome of wheat under HS. To conclude, TaCPK-1 plays important role in triggering signaling network and in modulation of HS-tolerance in wheat.
The online version contains supplementary material available at 10.1007/s13205-024-03989-6.
钙依赖蛋白激酶（CDPK）因其作为热传感器的特殊作用，是植物系统内最重要的信号级联之一的成员。在此，我们从小麦中鉴定出28个全长推定基因，命名为TaCPK（1 - 28）。基于数字基因表达，我们克隆了全长为1691个核苷酸的TaCPK - 1基因，其开放阅读框（ORF）为548个氨基酸（登录号OP125853）。与对照相比，在热胁迫（38 ± 3°C，2小时）下，TaCPK - 1在耐热小麦HD2985的叶片中表达量最高（3.1倍）。观察到TaCPK - 1的表达与其他参与耐热性的胁迫相关基因（TaHSP17.6、TaHSP20.1、TaHSP20.2、TaHSP20.3、TaHSP20.4、TaHSP20.5、TaHSP20.6和TaHSP20.7）之间存在正相关。整体蛋白激酶分析表明，在热胁迫下，与根、茎和穗相比，叶片中的活性最高。免疫印迹分析表明，与热敏感的HD2329相比，耐热小麦HD2985在热胁迫（38 ± 3°C，2小时）下CDPK蛋白含量丰富。钙离子（Ca）作为CDPK的诱导剂，在耐热小麦HD2985的叶片组织（Ca - 622 ppm）中在热胁迫下显示出强烈的钙信号特征，而在穗组织中含量最低（Ca - 201 ppm）。我们观察到热胁迫下小麦离子组存在显著差异。总之，TaCPK - 1在触发信号网络和调节小麦耐热性方面发挥重要作用。
在线版本包含可在10.1007/s13205 - 024 - 03989 - 6获取的补充材料。
