Department of Biological Engineering, Inha University, Incheon, 402-751, Korea.
Sci Rep. 2017 Dec 1;7(1):16720. doi: 10.1038/s41598-017-16814-3.
Since there are several casualties due to uncontrolled bleeding resulting from simple injury to surgery, effective styptic or vessel adhesives are important; however, their development is limited by the lack of standardized systems to evaluate potential compounds. The current study outlines the development of an aorta styptic evaluation system, comprising of decellularized swine aorta tissue and a heart pump-mimicking system. Although the cells in the swine aorta were removed, the structural stability of the aorta was sustained due to the maintenance of the extracellular matrix. Using a control adhesive, Cyanoacrylate, the developed model was found to have similar adhesive efficacy to intact aorta. The circulatory-mimicking system was designed to mimic the beat rate and strength of blood-flow from the heart, which was necessary to evaluate the adherent efficacy. The decellularized aorta improves instabilities of intact tissues, which occurs on account of storage and origin, thereby allowing for a more standardized system. The system was able to simulate several symptoms of circulation, according to patient age and health, by adjusting pumping frequency and intensity. Therefore, this system can be used as a standardized evaluation system for screening adhesives. Further, it would also evaluate other medical devices, such as stent or medications.
由于简单的创伤或手术导致的无法控制的出血而导致了几个人伤亡，有效的止血剂或血管黏合剂非常重要；然而，由于缺乏标准化的系统来评估潜在的化合物，它们的发展受到限制。本研究概述了一种主动脉止血评估系统的开发，该系统由脱细胞猪主动脉组织和心脏泵模拟系统组成。尽管猪主动脉中的细胞已被去除，但由于细胞外基质的维持，主动脉的结构稳定性得以维持。使用氰基丙烯酸酯作为对照黏合剂，发现所开发的模型具有与完整主动脉相似的黏附效果。循环模拟系统的设计旨在模拟心脏的搏动率和血流强度，这对于评估黏附效果是必要的。脱细胞的主动脉改善了完整组织的不稳定性，这种不稳定性是由于组织的储存和来源造成的，从而使系统更加标准化。该系统可以通过调整泵送频率和强度来模拟根据患者年龄和健康状况出现的几种循环症状。因此，该系统可以用作筛选黏合剂的标准化评估系统。此外，它还可以评估其他医疗设备，如支架或药物。
