Thibeault Andrée-Anne Hudon, Sanderson J Thomas, Vaillancourt Cathy
INRS - Institut Armand-Frappier, 531, Boulevard des Prairies, Laval, QC, Canada, H7V 1B7.
BioMed Research Centre, Université du Québec à Montréal, C.P. 8888, succ. Centre-ville, Montréal, QC, Canada, H3C 3P8.
Methods Mol Biol. 2018;1710:295-304. doi: 10.1007/978-1-4939-7498-6_23.
Estrogens are produced in large amounts during pregnancy, as a result of a tightly regulated cooperation between the maternal and fetal adrenal cortex, which produce androgen precursors, and the placental villous trophoblast, which transforms these precursors into estrogens. These estrogens play an important role in proper placental function, in adaptation of the mother to pregnancy, as well as in adequate fetal development. Disruption of estrogen production is associated with poor pregnancy outcomes and fetal malformation or altered fetal programming. Pregnant women may be exposed to endocrine disruptors from environmental sources or medications, and it is crucial to study the effects of such compounds on feto-placental steroidogenesis. The H295R/BeWo co-culture model offers the opportunity to study these interactions, by making it possible to evaluate the effects of chemical exposures on androgen and estrogen biosynthesis, as well as on various other aspects of feto-placental communication.
雌激素在孕期大量产生，这是由于母体和胎儿肾上腺皮质（产生雄激素前体）与胎盘绒毛滋养层（将这些前体转化为雌激素）之间严格调控的协作所致。这些雌激素在胎盘正常功能、母亲对妊娠的适应以及胎儿的充分发育中发挥着重要作用。雌激素生成的中断与不良妊娠结局、胎儿畸形或胎儿编程改变有关。孕妇可能会接触到来自环境源或药物的内分泌干扰物，研究此类化合物对胎儿 - 胎盘类固醇生成的影响至关重要。H295R/BeWo共培养模型提供了研究这些相互作用的机会，因为它能够评估化学暴露对雄激素和雌激素生物合成以及胎儿 - 胎盘通讯的其他各个方面的影响。
