INRS - Institut Armand-Frappier, Laval, QC, H7V 1B7, Canada.
Reprod Toxicol. 2019 Dec;90:33-43. doi: 10.1016/j.reprotox.2019.08.010. Epub 2019 Aug 16.
We determined whether 5 common essential oils (basil, fennel seed, orange, black pepper and sage) interfered with feto-placental steroidogenesis in a co-culture model composed of fetal-like adrenocortical (H295R) and placental trophoblast-like (BeWo) cells. After a 24 h exposure, only basil and fennel seed oil significantly increased hormone concentrations of estradiol, estrone, dehydroepiandrosterone (DHEA), androstenedione, progesterone, and estriol. Basil and fennel seed oil were shown to significantly alter the expression of steroidogenic enzymes involved in cholesterol transport and steroid hormone biosynthesis, including StAR, CYP11A1, 3β-HSD1/2, SULT2A1, and HSD17β1, -4, and -5. Also, basil and fennel seed oil stimulated placental-specific promoter I.1 and pII-derived CYP19 mRNA in BeWo and H295R cells, respectively, as well as, increased CYP19 enzyme activity. Our results indicate that further study is necessary to determine the potential risks of using basil and fennel seed oils during pregnancy considering their potential to disrupt steroidogenic enzyme activity and expression in vitro.
我们研究了 5 种常见的精油（罗勒、茴香籽、橙、黑胡椒和鼠尾草）是否会在由胎儿样肾上腺皮质（H295R）和胎盘滋养层样（BeWo）细胞组成的共培养模型中干扰胎儿胎盘类固醇生成。经过 24 小时暴露，只有罗勒和茴香籽油显著增加了雌二醇、雌酮、脱氢表雄酮（DHEA）、雄烯二酮、孕酮和雌三醇的激素浓度。罗勒和茴香籽油被证明显著改变了参与胆固醇转运和类固醇激素生物合成的类固醇生成酶的表达，包括 StAR、CYP11A1、3β-HSD1/2、SULT2A1 和 HSD17β1、-4 和 -5。此外，罗勒和茴香籽油分别刺激 BeWo 和 H295R 细胞中胎盘特异性启动子 I.1 和 pII 衍生的 CYP19 mRNA，以及增加 CYP19 酶活性。我们的研究结果表明，鉴于它们在体外干扰类固醇生成酶活性和表达的潜力，有必要进一步研究在怀孕期间使用罗勒和茴香籽油的潜在风险。
