Reynaud Déborah, Sergent Frédéric, Abi Nahed Roland, Brouillet Sophie, Benharouga Mohamed, Alfaidy Nadia
Unité U1036, Institut National de la Santé et de la Recherche Médicale, Grenoble, France.
University Grenoble-Alpes, 38000, Grenoble, France.
Methods Mol Biol. 2018;1710:317-324. doi: 10.1007/978-1-4939-7498-6_25.
During the last decade, multiple animal models have been developed to mimic hallmarks of pregnancy-induced hypertension (PIH) diseases, which include gestational hypertension, preeclampsia (PE), or eclampsia. Converging in vitro, ex vivo, and clinical studies from our group strongly suggested the potential involvement of the new angiogenic factor EG-VEGF (endocrine gland-derived-VEGF) in the development of PIH. Here, we described the protocol that served to demonstrate that maintenance of EG-VEGF production over 11.5 days post coitus (dpc) in the gravid mice caused the development of PIH. The developed model exhibited most hallmarks of preeclampsia.
在过去十年中，已经开发出多种动物模型来模拟妊娠高血压疾病（PIH）的特征，其中包括妊娠高血压、子痫前期（PE）或子痫。我们团队进行的体外、离体和临床研究一致有力地表明，新的血管生成因子EG-VEGF（内分泌腺源性VEGF）可能参与了PIH的发展。在此，我们描述了一个实验方案，该方案用于证明妊娠小鼠在交配后11.5天（dpc）以上维持EG-VEGF的产生会导致PIH的发生。所建立的模型表现出子痫前期的大多数特征。
