光学相干断层扫描和计算机辅助诊断慢性肾脏病小鼠模型。
Optical coherence tomography and computer-aided diagnosis of a murine model of chronic kidney disease.
机构信息
University of Maryland, Department of Electrical and Computer Engineering, College Park, Maryland, United States.
University of Maryland, Fischell Department of Bioengineering, College Park, Maryland, United States.
出版信息
J Biomed Opt. 2017 Dec;22(12):1-11. doi: 10.1117/1.JBO.22.12.121706.
Chronic kidney disease (CKD) is characterized by a progressive loss of renal function over time. Histopathological analysis of the condition of glomeruli and the proximal convolutional tubules over time can provide valuable insights into the progression of CKD. Optical coherence tomography (OCT) is a technology that can analyze the microscopic structures of a kidney in a nondestructive manner. Recently, we have shown that OCT can provide real-time imaging of kidney microstructures in vivo without administering exogenous contrast agents. A murine model of CKD induced by intravenous Adriamycin (ADR) injection is evaluated by OCT. OCT images of the rat kidneys have been captured every week up to eight weeks. Tubular diameter and hypertrophic tubule population of the kidneys at multiple time points after ADR injection have been evaluated through a fully automated computer-vision system. Results revealed that mean tubular diameter and hypertrophic tubule population increase with time in post-ADR injection period. The results suggest that OCT images of the kidney contain abundant information about kidney histopathology. Fully automated computer-aided diagnosis based on OCT has the potential for clinical evaluation of CKD conditions.
慢性肾脏病（CKD）的特征是肾功能随时间逐渐丧失。对肾小球和近端浓缩管随时间变化的组织病理学分析可以深入了解 CKD 的进展。光学相干断层扫描（OCT）是一种可以非破坏性地分析肾脏微观结构的技术。最近，我们已经证明，OCT 可以在不使用外源性造影剂的情况下实时成像活体肾脏的微观结构。通过 OCT 评估静脉注射阿霉素（ADR）诱导的 CKD 小鼠模型。在 8 周内每周对大鼠肾脏进行 OCT 图像采集。通过全自动计算机视觉系统评估 ADR 注射后多个时间点的肾脏管腔直径和肥大管腔数量。结果表明，ADR 注射后，平均管腔直径和肥大管腔数量随时间增加。结果表明，肾脏 OCT 图像包含有关肾脏组织病理学的丰富信息。基于 OCT 的全自动计算机辅助诊断有可能用于 CKD 状况的临床评估。
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