Tang Qinggong, Wang Jianting, Frank Aaron, Lin Jonathan, Li Zhifang, Chen Chao-Wei, Jin Lily, Wu Tongtong, Greenwald Bruce D, Mashimo Hiroshi, Chen Yu
Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA; Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou 350007, China.
Biomed Opt Express. 2016 Nov 21;7(12):5218-5232. doi: 10.1364/BOE.7.005218. eCollection 2016 Dec 1.
Early detection of neoplastic changes remains a critical challenge in clinical cancer diagnosis and treatment. Many cancers arise from epithelial layers such as those of the gastrointestinal (GI) tract. Current standard endoscopic technology is difficult to detect the subsurface lesions. In this research, we investigated the feasibility of a novel multi-modal optical imaging approach including high-resolution optical coherence tomography (OCT) and high-sensitivity fluorescence laminar optical tomography (FLOT) for structural and molecular imaging. The C57BL/6J-Apc/J mice were imaged using OCT and FLOT, and the correlated histopathological diagnosis was obtained. Quantitative structural (scattering coefficient) and molecular (relative enzyme activity) parameters were obtained from OCT and FLOT images for multi-parametric analysis. This multi-modal imaging method has demonstrated the feasibility for more accurate diagnosis with 88.23% (82.35%) for sensitivity (specificity) compared to either modality alone. This study suggested that combining OCT and FLOT is promising for subsurface cancer detection, diagnosis, and characterization.
肿瘤性病变的早期检测仍然是临床癌症诊断和治疗中的一项关键挑战。许多癌症起源于上皮层，如胃肠道（GI）的上皮层。当前的标准内镜技术难以检测到黏膜下病变。在本研究中，我们研究了一种新型多模态光学成像方法的可行性，该方法包括用于结构和分子成像的高分辨率光学相干断层扫描（OCT）和高灵敏度荧光层状光学断层扫描（FLOT）。使用OCT和FLOT对C57BL/6J-Apc/J小鼠进行成像，并获得相关的组织病理学诊断。从OCT和FLOT图像中获取定量结构（散射系数）和分子（相对酶活性）参数进行多参数分析。与单独使用任何一种模态相比，这种多模态成像方法已证明更准确诊断的可行性，灵敏度为88.23%（特异性为82.35%）。这项研究表明，将OCT和FLOT相结合在黏膜下癌症检测、诊断和表征方面具有前景。
