Fajkić Almir, Musić Miralem, Kulenović Alma Džubur, Začiragić Asija, Avdagić Nesina, Babić Nermina, Lepara Orhan, Pepić Esad, Dervišević Amela, Huskić Jasminko
Department of Pathophysiology, Medical Faculty, University of Sarajevo, Čekalusa 90, 71000 Sarajevo, Bosnia and Herzegovina,
Psychiatr Danub. 2017 Dec;29(4):431-436. doi: 10.24869/psyd.2017.431.
The aim of the present study was to evaluate serum nitric oxide (NO) and C reactive protein (CRP) concentration in veterans with and without PTSD. Furthermore, we aimed to assess whether there is a correlation between serum NO and CRP concentrations in tested groups.
Cross-sectional study included 90 male individuals, with and without experience of direct war combat, divided into three equal groups (n=30): group 1- included war veterans with PTSD, group 2 - included war veterans without PTSD, and control group - 30 apparently healthy volunteers, without experience of direct war combat. The diagnosis of PTSD was assessed according to the guidelines in the 10 revision of the International Classification of Diseases (ICD-10). High-sensitivity CRP was determined by immunonephelometry. The serum NO level was determined by classic colorimetrical Griess reaction.
Serum CRP concentration in veterans with (3.54±1.19 mg/L) and without PTSD (3.24±2.04 mg/L), was significantly higher (p<0.05) compared to control group (1.26±1.06 mg/L). Serum NO concentration in veterans with (7.64±4.43 μmol/L) and without PTSD (7.12±2.60 μmol/L) was significantly lower (p<0.05) compared to control group (11.26±7.01 μmol/L). Statistically significant correlation between serum NO and CRP concentration was determined in veterans without PTSD (r=-0.473; p<0.01). No correlation was observed between serum NO and CRP concentration in veterans with PTSD (r=0.118; p=0.534) and in control group (r=-0.067; p=0.727).
The present study has showed significant increase of serum CRP and significant decrease of serum NO concentrations in veterans with and without PTSD. Furthermore, statistically significant negative correlation between serum NO and CRP concentration was determined only in veterans without PTSD. Obtained results indicate that the complex mechanism of the pathogenesis of PTSD requires further research.
本研究旨在评估患有和未患有创伤后应激障碍（PTSD）的退伍军人血清一氧化氮（NO）和C反应蛋白（CRP）浓度。此外，我们旨在评估受试组中血清NO与CRP浓度之间是否存在相关性。
横断面研究纳入了90名男性个体，有或无直接战争经历，分为三个相等的组（n = 30）：第1组 - 包括患有PTSD的退伍军人，第2组 - 包括未患有PTSD的退伍军人，对照组 - 30名明显健康的志愿者，无直接战争经历。根据《国际疾病分类》第10版（ICD - 10）中的指南评估PTSD的诊断。通过免疫比浊法测定高敏CRP。血清NO水平通过经典的比色Griess反应测定。
患有PTSD的退伍军人血清CRP浓度（3.54±1.19 mg/L）和未患有PTSD的退伍军人血清CRP浓度（3.24±2.04 mg/L），与对照组（1.26±1.06 mg/L）相比显著更高（p < 0.05）。患有PTSD的退伍军人血清NO浓度（7.64±4.43 μmol/L）和未患有PTSD的退伍军人血清NO浓度（7.12±2.60 μmol/L），与对照组（11.26±7.01 μmol/L）相比显著更低（p < 0.05）。在未患有PTSD的退伍军人中确定血清NO与CRP浓度之间存在统计学显著相关性（r = -0.473；p < 0.01）。在患有PTSD的退伍军人中（r = 0.118；p = 0.534）和对照组中（r = -0.067；p = 0.727）未观察到血清NO与CRP浓度之间的相关性。
本研究表明，患有和未患有PTSD的退伍军人血清CRP显著升高，血清NO浓度显著降低。此外，仅在未患有PTSD的退伍军人中确定血清NO与CRP浓度之间存在统计学显著的负相关性。获得的结果表明，PTSD发病机制的复杂机制需要进一步研究。
