人绒毛膜促性腺激素在体外对睾丸间质细胞芳香化作用的急性刺激。
Acute stimulation of aromatization in Leydig cells by human chorionic gonadotropin in vitro.
作者信息
Valladares L E, Payne A H
出版信息
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4460-3. doi: 10.1073/pnas.76.9.4460.
Abstract
Aromatization of testosterone in Leydig cells purified from mature rat testes was assessed. Leydig cells incubated for 4 hr with increasing concentrations of [3H]testosterone. At saturating concentrations of testosterone, human chorionic gonadotropin (hCG) acutely stimulated aromatization. This stimulation was first observed at 1 hr, an 8-fold increase being found during a 4-hr incubation. The maximal amount of estradiol produced at saturating concentrations of testosterone and hCG was 1.8 ng per 10(6) cells. These results demonstrate that purified Leydig cells have a high capacity for aromatization and that hCG can acutely stimulate aromatization independently of stimulating testosterone synthesis in vitro.
摘要
对从成熟大鼠睾丸中纯化的睾丸间质细胞中睾酮的芳香化作用进行了评估。将睾丸间质细胞与浓度不断增加的[3H]睾酮孵育4小时。在睾酮达到饱和浓度时，人绒毛膜促性腺激素（hCG）可急性刺激芳香化作用。这种刺激在1小时时首次观察到，在4小时的孵育过程中发现增加了8倍。在睾酮和hCG达到饱和浓度时产生的雌二醇最大量为每10(6)个细胞1.8纳克。这些结果表明，纯化的睾丸间质细胞具有很高的芳香化能力，并且hCG在体外可独立于刺激睾酮合成而急性刺激芳香化作用。
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