Payne A H, Kelch R P, Musich S S, Halpern M E
J Clin Endocrinol Metab. 1976 Jun;42(6):1081-7. doi: 10.1210/jcem-42-6-1081.
To determine the intratesticular site of aromatization, homogenates of separated seminiferous tubules and intact tissue from human testes were incubated with [3H] androstenedione or [3H] testosterone. [3H] Estrogens were isolated and identified, and the amounts synthesized were expressed as pmol/mg protein incubated. In testicular tissue from the three adult subjects investigated, the total estrogen per mg protein was 1.5-2.7 times greater in intact tissue than in isolated seminiferous tubules. This suggests that the major site of aromatization in human testes is the interstitial tissue. Estradiol was by far the major estrogenic product. No differences were observed in the amount of estrogens synthesized whether the substrate was androstenedione or testosterone.
为了确定睾丸内芳香化作用的位点，将来自人类睾丸的分离生精小管和完整组织的匀浆与[3H]雄烯二酮或[3H]睾酮一起孵育。分离并鉴定了[3H]雌激素，合成量以每毫克孵育蛋白的皮摩尔数表示。在所研究的三名成年受试者的睾丸组织中，每毫克蛋白的总雌激素量在完整组织中比在分离的生精小管中高1.5至2.7倍。这表明人类睾丸中芳香化作用的主要位点是间质组织。雌二醇是迄今为止主要的雌激素产物。无论底物是雄烯二酮还是睾酮，所合成的雌激素量均未观察到差异。
