从人脑中纯化及鉴定一种半乳糖苷结合凝集素

Purification and characterization of a galactoside-binding lectin from human brain.

作者信息

Bladier D, Joubert R, Avellana-Adalid V, Kémény J L, Doinel C, Amouroux J, Caron M

机构信息

Laboratoire de Biochimie et Technologie des Protéines, UFR Santé, Médecine et Biologie Humaine, Bobigny, France.

出版信息

Arch Biochem Biophys. 1989 Mar;269(2):433-9. doi: 10.1016/0003-9861(89)90127-6.

DOI:10.1016/0003-9861(89)90127-6

PMID:2919877

Abstract

A beta-galactoside-binding hemagglutinin was detected in soluble extracts of human brain. This soluble lectin was purified to homogeneity by affinity column chromatography on lactose coupled to divinylsulfone-activated agarose. The purified lectin had an isoelectric point of 3.9 and its subunit molecular mass estimated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate was 14,500. Human brain lectin was not a glycoprotein and its amino acid composition was characterized by a high content of serine, glutamic acid, and glycine, and a low content of methionine and cysteine. The most potent saccharide inhibitors tested were thiodigalactoside, lactose, and p-nitrophenyl-beta-D-galactoside. An antibody was raised to the pure lectin. Immunological relationships were found between the brain lectin and several other soluble lectins of various vertebrate origins.

摘要

在人脑的可溶性提取物中检测到一种β-半乳糖苷结合血凝素。这种可溶性凝集素通过在与二乙烯砜活化琼脂糖偶联的乳糖上进行亲和柱色谱纯化至同质。纯化后的凝集素的等电点为3.9，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳估计其亚基分子量为14,500。人脑凝集素不是糖蛋白，其氨基酸组成的特点是丝氨酸、谷氨酸和甘氨酸含量高，蛋氨酸和半胱氨酸含量低。测试的最有效的糖类抑制剂是硫代二半乳糖苷、乳糖和对硝基苯基-β-D-半乳糖苷。制备了针对纯凝集素的抗体。发现脑凝集素与多种脊椎动物来源的其他几种可溶性凝集素之间存在免疫关系。