Paull D E, Keagy B A, Kron E J, Wilcox B R
Division of Cardiothoracic Surgery, University of North Carolina School of Medicine, Chapel Hill 27599-7065.
Ann Thorac Surg. 1989 Feb;47(2):187-92. doi: 10.1016/0003-4975(89)90267-1.
The left lower lobes of 28 canine lungs were isolated, preserved, and then reperfused for 150 minutes. Five groups of lobes were studied: group 1, control (n = 5); group 2, one hour of warm ischemia (n = 5); group 3, one hour of warm ischemia + oxygen free radical scavengers (n = 5); group 4, 24 hours of cold ischemia (n = 8); and group 5, 24 hours of cold ischemia + oxygen free radical scavengers (n = 5). Oxygen free radical scavengers consisted of superoxide dismutase and catalase (100 micrograms/mL) given at the moment of reflow. Extravascular lung water (grams per gram of blood-free dry lobe weight) after reperfusion was 2.75 +/- 0.19, 5.46 +/- 0.60, 4.08 +/- 0.37, 9.43 +/- 0.98, and 6.91 +/- 0.95 for groups 1 through 5, respectively (p less than 0.05, groups 2 through 5 versus group 1; p less than 0.05, group 2 versus group 3 and group 4 versus group 5). Lung tissue lipid peroxidation, measured as thiobarbituric acid reactive material, was 117 +/- 14, 314 +/- 19, and 163 +/- 25 nmol/g dry lobe weight for groups 1, 4, and 5, respectively (p less than 0.05, group 4 versus group 1 and group 4 versus group 5). The data suggest that oxygen free radical scavengers attenuate reperfusion injury after long-term hypothermic lung preservation.
选取28只犬的左肺下叶进行分离、保存，然后再灌注150分钟。研究分为五组：第一组为对照组（n = 5）；第二组为1小时热缺血（n = 5）；第三组为1小时热缺血+氧自由基清除剂（n = 5）；第四组为24小时冷缺血（n = 8）；第五组为24小时冷缺血+氧自由基清除剂（n = 5）。氧自由基清除剂为超氧化物歧化酶和过氧化氢酶（100微克/毫升），在再灌注时给予。再灌注后血管外肺水（每克无血干肺叶重量的克数），第一组至第五组分别为2.75±0.19、5.46±0.60、4.08±0.37、9.43±0.98和6.91±0.95（第二组至第五组与第一组相比，p<0.05；第二组与第三组相比，第四组与第五组相比，p<0.05）。以硫代巴比妥酸反应物质衡量的肺组织脂质过氧化，第一组、第四组和第五组分别为117±14、314±19和163±25纳摩尔/克干肺叶重量（第四组与第一组相比，第四组与第五组相比，p<0.05）。数据表明，氧自由基清除剂可减轻长期低温肺保存后的再灌注损伤。
