Li Yuan, Zhou Jian-Wei, Li Hui-Liang, Liu Jin-Zhong
Department of Stomatology, Zhengzhou First People's Hospital. Zhengzhou 450004, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2017 Aug;26(4):409-413.
To investigate the expression of Smad4, Smad7 and Caveolin-1 in the process of carcinogenesis of oral mucosa in Wistar rats, and to understand the changes of TGF-β/Smad signaling pathway and Caveolin-1 in oral cancer.
Palatal mucosal carcinogenesis specimens of Wistar rats were obtained from School of Stomatology, Zhengzhou University, which included 5 samples of normal mucosa, 10 samples of simple hyperplasia mucosa, 6 samples of mild mucosal dysplasia, 7 samples of moderate mucosal dysplasia, 13 samples of mucosa severe mucosal dysplasia, and 28 samples of oral cancer tissue. The expression of Smad4, Smad7 and Caveolin-1 was detected by immunohistochemistry. SPSS15.0 software package was used for statistical analysis.
The expression of Smad4 decreased in normal and hyperplastic epithelia, dysplasticepithelia and oral cancer gradually, the difference of the expression among the three groups was significant (P<0.05). The expression of Smad7 and Caveolin-1 increased in normal and hyperplastic epithelia, dysplasticepithelia and oral cancer gradually, respectively; the difference of the expression among the 3 groups was significant (P<0.05). Spearman correlation analysis showed that Smad4 was negatively correlated with Smad7, Smad4 was negatively correlated with caveolin-1, Smad7 was positively correlated with Caveolin-1 (P<0.05).
Synergistic effects may exist among Smad4, Smad7 and caveolin-1 in carcinogenesis of oral cancer.
研究Smad4、Smad7和小窝蛋白-1在Wistar大鼠口腔黏膜癌变过程中的表达情况，了解TGF-β/Smad信号通路及小窝蛋白-1在口腔癌中的变化。
从郑州大学口腔医学院获取Wistar大鼠腭黏膜癌变标本，包括5例正常黏膜样本、10例单纯增生黏膜样本、6例轻度黏膜发育异常样本、7例中度黏膜发育异常样本、13例重度黏膜发育异常样本以及28例口腔癌组织样本。采用免疫组织化学法检测Smad4、Smad7和小窝蛋白-1的表达。使用SPSS15.0软件包进行统计分析。
Smad4在正常上皮、增生上皮、发育异常上皮及口腔癌中的表达逐渐降低，三组间表达差异有统计学意义（P<0.05）。Smad7和小窝蛋白-1分别在正常上皮、增生上皮、发育异常上皮及口腔癌中的表达逐渐升高；三组间表达差异有统计学意义（P<0.05）。Spearman相关性分析显示，Smad4与Smad7呈负相关，Smad4与小窝蛋白-1呈负相关，Smad7与小窝蛋白-1呈正相关（P<0.05）。
Smad4、Smad7和小窝蛋白-1在口腔癌发生过程中可能存在协同作用。
