Liu Duan-qin, Zhang Jun, Song Hong-ning, Zheng Jun, Wang Xu-xia
Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University. Jinan 250012;China.E-mail:
Shanghai Kou Qiang Yi Xue. 2013 Oct;22(5):492-7.
To observe the changes of the expressions of Smad4 and Smad7 in oral squamous cell carcinoma and adjacent normal tissue, and to investigate the effects of Smad4 and Smad7 on occurrence and development of oral squamous cell carcinoma (OSCC).
Seventy-two cases with OSCC which were pathologically confirmed were included in the study. Cancer tissue and adjacent normal tissue were taken to make slices. SP immunohistochemical method was used to detect the expression of Smad4 and Smad7 in oral squamous cell carcinoma and adjacent normal tissue. SPSS11.5 software package was used for statistical analysis.
The positive expression rate of Smad4 protein was 69.44% in adjacent normal tissues, and it was 45.83% in oral squamous cell carcinoma(P<0.05). The positive expression rate of Smad4 was lower when the degree of differentiation decreased; The positive expression rate of Smad4 with lymph node metastasis was 22.50%, while it was 65.63% without lymph node metastasis(P<0.05). The positive expression rate of Smad7 protein in adjacent normal tissues was 19.44%, and it was 83.33% (P<0.05) in oral squamous cell carcinoma, the positive expression rate of Smad7 was higher when the degree of differentiation decreased. The positive expression rate of Smad7 with lymph node metastasis was 92.50%, while it was 68.75%(P<0.05) without lymph node metastasis.
The expression of Smad4 is significantly reduced in cancer tissues and the expression is lower when the degree of differentiation decreased; the expression rate of Smad4 with lymph node metastasis is lower compared with those without lymph node metastasis. Smad7 has the opposite effect. Loss of expression of Smad4 may contribute to the development and metastasis of oral squamous cell carcinoma. Over-expression of Smad7 may promote the development and metastasis of oral squamous cell carcinoma. The inhibition of BMP/Smads signaling pathway may result in the occurrence and development of oral squamous cell carcinoma continually. Supported by Science and Technology Development Plan (2010GSF10239) of Shandong Province and Science and Technology Special Project of Shandong Province (2012G0021852).
观察Smad4和Smad7在口腔鳞状细胞癌及癌旁正常组织中的表达变化，探讨Smad4和Smad7对口腔鳞状细胞癌（OSCC）发生发展的影响。
选取72例经病理确诊的OSCC患者，取癌组织及癌旁正常组织制作切片，采用SP免疫组织化学方法检测口腔鳞状细胞癌及癌旁正常组织中Smad4和Smad7的表达，应用SPSS11.5软件包进行统计学分析。
Smad4蛋白在癌旁正常组织中的阳性表达率为69.44%，在口腔鳞状细胞癌中为45.83%（P<0.05）。Smad4阳性表达率随分化程度降低而降低；有淋巴结转移的Smad4阳性表达率为22.50%，无淋巴结转移的为65.63%（P<0.05）。Smad7蛋白在癌旁正常组织中的阳性表达率为19.44%，在口腔鳞状细胞癌中为83.33%（P<0.05），Smad7阳性表达率随分化程度降低而升高。有淋巴结转移的Smad7阳性表达率为92.50%，无淋巴结转移的为68.75%（P<0.05）。
Smad4在癌组织中的表达明显降低，且随分化程度降低而降低；有淋巴结转移的Smad4表达率低于无淋巴结转移者。Smad7则相反。Smad4表达缺失可能促进口腔鳞状细胞癌的发生发展，Smad7过表达可能促进口腔鳞状细胞癌的发生发展。BMP/Smads信号通路的抑制可能导致口腔鳞状细胞癌的持续发生发展。由山东省科技发展计划（2010GSF10239）和山东省科技专项（2012G0021852）资助。
