非小细胞肺癌（腺癌组织学类型）中表皮生长因子受体（EGFR）突变的发生率及特征：来自加尔各答的106例患者报告

Incidence and characteristics of Epidermal Growth Factor Receptor (EGFR) mutation in non-small-cell lung cancer (Adenocarcinoma histology): A report of 106 patients from Kolkata.

作者信息

Chatterjee K, Ray A, Chattopadhyay B

机构信息

Department of Radiotherapy, IPGMER & SSKM Hospitals, Kolkata, West Bengal, India.

Department of Radiotherapy, Ruby General Hospital, Kolkata, West Bengal, India.

出版信息

Indian J Cancer. 2017 Jan-Mar;54(1):305-307. doi: 10.4103/ijc.IJC_239_17.

DOI:10.4103/ijc.IJC_239_17

PMID:29199710

Abstract

CONTEXT

Regional epidemiological data regarding the epidermal growth factor receptor (EGFR) mutation status in non-small-cell lung cancers (NSCLC) is an unmet need from the eastern part of India.

AIMS

To report the incidence of EGFR mutation and its correlation with the phenotypical characteristics, in NSCLC patients from Kolkata.

SUBJECTS AND METHODS

NSCLC patients, with adenocarcinoma histology, whose tissues had been tested for EGFR mutational status between March 2014 and February 2017, were considered for this study. The testing methods used were Real-time-based amplification refractory mutation system, polymerase chain reaction (ARMS PCR), PCR and gene sequencing, and cell-free DNA (CTDNA). Clinical characteristics and treatment details were collected from the patient's medical records in a de-identified manner.

STATISTICAL ANALYSIS USED

Data were analyzed using simple descriptive statistical methods.

RESULTS

Between March 2014 and February 2017, 108 samples were tested and two were deemed inadequate for reporting. Of the remaining 106 patients, 65 (61.3%) were males, and 41 (38.6%) were females. Median age was 56 years (42-72), 59 years for males and 52 years for females. 73.6% were nonsmokers. 87.7% tests were done using the real-time ARMS-PCR; 9.4% underwent PCR and gene sequencing and 2.8% using CT-DNA. Of 106 patients, 35 (33%) patients were found to be EGFR mutation positive. Ratio of male:female was 16 (45.7%):19 (54.3%). Ratio of nonsmoker: smoker was 30 (85.7%):5 (14.3%). 18 patients had exon 19 deletion (51.4%), 15 had L858R exon 21 mutation (42.9%), 1 patient (2.9%) had mutation in S7681 exon 20 along with L858R 21 and one patient (2.9%) had a T790m mutation without any other detectable EGFR mutation.

CONCLUSIONS

The incidence of EGFR mutation in NSCLC is 33% from Kolkata and is typically more common in females and nonsmokers.

摘要

背景

印度东部地区缺乏关于非小细胞肺癌（NSCLC）中表皮生长因子受体（EGFR）突变状态的区域流行病学数据。

目的

报告加尔各答NSCLC患者中EGFR突变的发生率及其与表型特征的相关性。

研究对象与方法

本研究纳入2014年3月至2017年2月期间组织进行过EGFR突变状态检测的腺癌组织学类型的NSCLC患者。所采用的检测方法包括基于实时扩增阻滞突变系统的聚合酶链反应（ARMS PCR）、PCR和基因测序以及游离DNA（CTDNA）。以去识别化的方式从患者病历中收集临床特征和治疗细节。

所用统计分析方法

采用简单描述性统计方法分析数据。

结果

2014年3月至2017年2月期间，共检测了108份样本，其中两份因报告不充分而被排除。在其余106例患者中，男性65例（61.3%），女性41例（38.6%）。中位年龄为56岁（42 - 72岁），男性为59岁，女性为52岁。73.6%为非吸烟者。87.7%的检测采用实时ARMS-PCR；9.4%的患者接受了PCR和基因测序，2.8%的患者采用CT-DNA检测。在106例患者中，35例（33%）患者被发现EGFR突变阳性。男性与女性的比例为16例（45.7%）：19例（54.3%）。非吸烟者与吸烟者的比例为30例（85.7%）：5例（14.3%）。18例患者存在外显子19缺失（51.4%），15例存在外显子21的L858R突变（42.9%），1例（2.9%）患者在外显子20的S7681处与L858R 21同时发生突变，1例（2.9%）患者存在T790m突变且无任何其他可检测到的EGFR突变。