Faculty of Medicine and Pharmacy, Laboratory of Biomedical and Translational Research, 314397Sidi Mohamed Ben Abdellah University, Fez, Morocco.
Laboratory of Anatomic Pathology and Molecular Pathology, University Hospital Hassan II, 470521Sidi Mohamed Ben Abdellah University, Fez, Morocco.
Cancer Control. 2022 Jan-Dec;29:10732748221084930. doi: 10.1177/10732748221084930.
Our prospective study aims to define the correlation of (epidermal growth factor receptor) mutations with major histological subtypes of lung adenocarcinoma from resected and non-resected specimens, according to the WHO 2015 classification, in Moroccan North East Population.
mutations of 150 primary lung adenocarcinoma were performed using Real-Time PCR or SANGER sequencing. SPSS 21 was used to assess the relationship between histological subtypes of lung adenocarcinoma and mutation status.
25 mutations were detected in the series of 150 lung adenocarcinomas, most of which were found in cases with papillary, acinar, patterns than without these patterns and more frequently occurred in the cases without solid pattern than with this pattern. A significant correlation was observed between mutation and acinar (P = 0,024), papillary pattern (P = 0,003) and, negative association with a solid pattern (P < 0,001). In females, mutations were significantly correlated with the acinar pattern (P = 0,02), whereas in males with the papillary pattern (P = 0,01). Association between the histologic component and exon 19 deletions and exon 21 mutations were also evaluated and, we found a significant correlation between the papillary major pattern with exon 19 mutations (P = 0,004) and, ex21 with the acinar component (P = 0,03).
An analysis of resected and non-resected lung ADC specimens in 150 Moroccan Northeast patients, revealed that acinar and papillary patterns may predict the presence of a mutation in the gene. While the solid major pattern may indicate a low mutation rate of the gene.
我们的前瞻性研究旨在根据 2015 年世界卫生组织分类，定义（表皮生长因子受体）突变与从切除和未切除标本中分离的肺腺癌主要组织学亚型之间的相关性，在摩洛哥东北地区人群中。
使用实时 PCR 或 SANGER 测序对 150 例原发性肺腺癌进行 突变检测。使用 SPSS 21 评估肺腺癌的组织学亚型与 突变状态之间的关系。
在 150 例肺腺癌系列中检测到 25 种突变，其中大多数在具有乳头状、腺泡样形态的病例中发现，而在没有这些形态的病例中更为常见，并且在没有实体形态的病例中比具有这种形态的病例更频繁发生。在统计学上， 突变与腺泡（P = 0.024）、乳头状模式（P = 0.003）显著相关，与实体模式呈负相关（P < 0.001）。在女性中， 突变与腺泡模式显著相关（P = 0.02），而在男性中与乳头状模式相关（P = 0.01）。还评估了组织学成分与外显子 19 缺失和外显子 21 突变之间的关联，我们发现主要的乳头状模式与外显子 19 突变（P = 0.004）和外显子 21 与腺泡成分之间存在显著相关性（P = 0.03）。
对 150 例摩洛哥东北部患者的切除和未切除肺 ADC 标本进行分析，显示腺泡和乳头状模式可能预测 基因的存在突变。而实体主要模式可能表明 基因的突变率较低。
