Crompton I E, Waley S G
Sir William Dunn School of Pathology, University of Oxford, U.K.
Biochem J. 1989 Jan 1;257(1):305-8. doi: 10.1042/bj2570305.
Extrapolation procedures are shown to be useful for obtaining kinetic parameters from irreversible enzymic reactions in which there are two intermediates, under both single-turnover and steady-state conditions. Small excesses of one component are treated as if they were large excesses, which is convenient in practice. The method has also been applied to a non-enzymic reversible bimolecular reaction.
结果表明,外推法对于在单周转和稳态条件下从具有两个中间体的不可逆酶促反应中获取动力学参数是有用的。将一种组分的少量过量视为大量过量,这在实际操作中很方便。该方法也已应用于非酶促可逆双分子反应。