[Stationary kinetics of multisubstrate enzymatic reactions. Inhibition by reaction products, reversible and irreversible inhibitors].

A generalized scheme of ordered multisubstrate enzymatic reactions under steady-state conditions is described. The equations for the reaction rate and product yield as well as the observed rate constant for the enzyme inactivation as functions of concentrations of substrates, reaction products, reversible and irreversible inhibitors were derived and analyzed. It was demonstrated that the kinetic behaviour of such systems can adequately be described in terms of interrelationship of intermediate forms of the enzyme.