Department of Chemistry, Center for Micro/Nano Science and Technology, and Advanced Optoelectronic Technology Center, National Cheng Kung University , Tainan 701, Taiwan.
National Nano Device Laboratories, National Applied Research Laboratories , Tainan 701, Taiwan.
ACS Appl Mater Interfaces. 2018 Jan 17;10(2):1508-1519. doi: 10.1021/acsami.7b14593. Epub 2018 Jan 3.
Construction of multifunctional nanoparticles (NPs) with near-infrared (NIR) plasmonic responses is considered a versatile and multifaceted platform for several biomedical applications. Herein, a double layer of Au/Ag alloy on the surface of truncated octahedral iron oxide NPs (IONPs) was prepared and the distance between the layers was controlled to exhibit broad and strong NIR absorption. The rattle-shaped IONP@shell-in-shell nanostructure showed light-response to the NIR biological window from 650 to 1300 nm for photothermal therapy (PTT) and magnetic guidance for hyperthermia and magnetic resonance imaging (MRI) diagnosis. Exposing the aqueous solution of IONP@shell-in-shell to a 1064 nm diode laser, its heat conversion efficiency was ∼28.3%. The in vitro cell viability at a gold concentration of 100 ppm was ∼85%, and decreased to ∼16% when the cells were treated with the NIR irradiation and magnetic attraction. T-weighted MRI images showed a clear accumulation of IONP@shell-in-shell at the tumor site with magnetic attraction. In vivo luminescence tumor images explained that the IONP@shell-in-shell could reduce the U87MG-luc2 cancer cell proliferation in mice with the NIR irradiation and magnetic attraction. These results indicate the IONP@shell-in-shell as a promising nanomedicine for PTT, magnetic targeting, and magnetic resonance imaging (MRI).
具有近红外（NIR）等离子体响应的多功能纳米粒子（NPs）的构建被认为是用于多种生物医学应用的多功能和多方面的平台。在此，在截角八面体氧化铁 NPs（IONPs）的表面制备了双层 Au/Ag 合金，并且控制层之间的距离以表现出宽且强的 NIR 吸收。这种笼中笼结构的纳米结构表现出对 NIR 生物窗口（650 至 1300nm）的光响应，可用于光热治疗（PTT）和磁引导的过热以及磁共振成像（MRI）诊断。将 IONP@shell-in-shell 的水溶液暴露于 1064nm 二极管激光下，其热转换效率约为 28.3％。在金浓度为 100ppm 时，体外细胞活力约为 85％，而在用 NIR 照射和磁吸引处理后，细胞活力降低至约 16％。T 加权 MRI 图像显示出在肿瘤部位有明显的 IONP@shell-in-shell 积累，并具有磁吸引作用。体内发光肿瘤图像表明，IONP@shell-in-shell 可以减少 U87MG-luc2 癌细胞在小鼠中的增殖，而无需 NIR 照射和磁吸引。这些结果表明 IONP@shell-in-shell 是一种很有前途的用于 PTT、磁靶向和磁共振成像（MRI）的纳米医学。
