Centre for Nanotechnology, ‡Department of Chemistry, and §Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati , Guwahati 781 039, India.
ACS Appl Mater Interfaces. 2018 Jan 31;10(4):3210-3218. doi: 10.1021/acsami.7b13782. Epub 2018 Jan 18.
A phenylboronic acid templated gold nanocluster probe was developed to detect biomarker mucin by a noninvasive fluorescence-based method using a point-of-care smartphone-based fluorescence detection device. The gold nanocluster probe is able to detect mucin specifically. The same probe was applied for in vitro targeted bioimaging of HeLa and Hep G2 cancer cells, and it demonstrated specific therapeutic effects toward cancer cells as well as multicellular tumor spheroids imparting theranostic properties. The module is found to be more effective toward HeLa cells, and a pathway of cell death was established using flow-cytometry-based assays.
开发了一种基于金纳米簇探针的苯硼酸模板，通过一种基于非侵入性荧光的方法，使用即时护理智能手机荧光检测设备来检测生物标志物粘蛋白。该金纳米簇探针能够特异性地检测粘蛋白。同样的探针也被应用于 HeLa 和 Hep G2 癌细胞的体外靶向生物成像，并且对癌细胞以及赋予治疗和诊断特性的多细胞肿瘤球也显示出了特定的治疗效果。该模块对 HeLa 细胞更有效，并且使用基于流式细胞术的测定法建立了细胞死亡途径。
