促分泌素诱导单个胰腺腺泡细胞中的钙动员。

Secretagogue induced calcium mobilization in single pancreatic acinar cells.

作者信息

Stuenkel E L, Tsunoda Y, Williams J A

机构信息

Department of Physiology, University of Michigan, Ann Arbor 48109.

出版信息

Biochem Biophys Res Commun. 1989 Feb 15;158(3):863-9. doi: 10.1016/0006-291x(89)92802-7.

DOI:10.1016/0006-291x(89)92802-7

PMID:2920043

Abstract

Microspectrofluorometry of fura-2 was utilized to monitor [Ca2+]i in single acinar cells stimulated with a cholinergic agonist and cholecystokinin. A similar amplitude of agonist induced Ca mobilization between single cell and populational approaches was observed. New findings in single cells not observable in populations of cells include: 1) the maintenance of a sustained elevation in [Ca2+]i above basal levels throughout agonist application, 2) the reloading of the agonist-sensitive Ca pool only following removal of the agonist and 3) the presence of oscillations of [Ca2+]i in response to agonist application which is enhanced at lower agonist concentrations.

摘要

利用fura-2的显微分光荧光测定法监测用胆碱能激动剂和胆囊收缩素刺激的单个腺泡细胞中的[Ca2+]i。观察到在单细胞和群体方法之间激动剂诱导的钙动员幅度相似。在单个细胞中可观察到而在细胞群体中未观察到的新发现包括：1）在整个激动剂应用过程中，[Ca2+]i维持在高于基础水平的持续升高状态；2）仅在去除激动剂后，激动剂敏感钙库才重新加载；3）响应激动剂应用时[Ca2+]i存在振荡，在较低激动剂浓度下增强。