Afzal Samina, Chaudhry Bashir Ahmad, Ahmad Ashfaq, Uzair Muhammad, Afzal Khurram
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan.
Department of Pharmacology and Toxicology, School of Medicine, Virginia Commonwealth University, Richmond, USA.
Pharmacogn Mag. 2017 Oct-Dec;13(52):647-651. doi: 10.4103/pm.pm_95_17. Epub 2017 Nov 13.
() commonly known as Boa-phalee belonging to the family Tiliaceae having 50 genera and 450 species. is not among the studied medicinal agent despite its potential in ethnopharmacology.
The present study investigated antioxidant, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glucosidase inhibitory activities of . The dichloromethane and methanolic extracts of the were evaluated for biological activities such as antioxidant and enzyme inhibitory activities of AChE, BChE, and α-glucosidase.
Antioxidant activity was evaluated by measuring free radical scavenging potential of using 1,1-diphenyl-2-picrylhydrazyl. Enzyme inhibition activities were done by measuring optical density.
The methanol extract of roots of showed potential free radical scavenging activity 99% at concentration 16.1 μg/ml. AChE was inhibited by aerial part of dichloromethane fraction by 46.07% ± 0.45% while dichloromethane extracts of roots of possessed significant activity against BChE with 86% inhibition compared with standard drug Eserine at concentration 0.5 mg/ml. The dichloromethane extract of roots of showed 79% inhibition against α-glucosidase enzyme activity with IC 62.8 ± 1.5 μg/ml.
These findings suggest as useful therapeutic option as antioxidant and inhibition of AChE, BChE, and α-glucosidase activities.
The aerial parts and roots of () were extracted in dichloromethane and methanolThe extract of roots of showed free radical scavenging activity 99% at concentration 16.1 mg/ml, Ach inhibition by aerial parts of dichloromethane fraction by 46.07%, and 79% inhibition against a-glucosidase enzyme activity with IC 62.8 ± 1.5 mg/mlThe dichloromethane and methanolic extracts of exhibited antioxidant inhibition of acetyl cholinesterase, butyrylcholinesterase, and a-glucosidase activities. DPPH: 1,1-diphenyl-2-picrylhydrazyl, : , AChE: Acetylcholinesterase, BChE: Butyrylcholinesterase, AD: Alzheimer's disease.
（）通常被称为Boa - phalee，属于椴树科，该科有50个属和450个物种。尽管其在民族药理学方面具有潜力，但它并不在已研究的药用剂之中。
本研究调查了（）的抗氧化、乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）和α - 葡萄糖苷酶抑制活性。对（）的二氯甲烷和甲醇提取物进行了生物活性评估，如AChE、BChE和α - 葡萄糖苷酶的抗氧化和酶抑制活性。
通过使用1,1 - 二苯基 - 2 - 苦基肼测定（）的自由基清除潜力来评估抗氧化活性。通过测量光密度来进行酶抑制活性测定。
（）根的甲醇提取物在浓度为16.1μg/ml时显示出99%的潜在自由基清除活性。二氯甲烷部分的地上部分对AChE的抑制率为46.07%±0.45%，而（）根的二氯甲烷提取物对BChE具有显著活性，与浓度为0.5mg/ml的标准药物毒扁豆碱相比，抑制率为86%。（）根的二氯甲烷提取物对α - 葡萄糖苷酶活性的抑制率为79%，IC为62.8±1.5μg/ml。
这些发现表明（）作为抗氧化剂以及抑制AChE、BChE和α - 葡萄糖苷酶活性是一种有用的治疗选择。
（）的地上部分和根用二氯甲烷和甲醇进行了提取。（）根的提取物在浓度为16.1mg/ml时显示出99%的自由基清除活性，二氯甲烷部分的地上部分对ACh的抑制率为46.07%，对α - 葡萄糖苷酶活性的抑制率为79%且IC为62.8±1.5mg/ml。（）的二氯甲烷和甲醇提取物表现出对乙酰胆碱酯酶、丁酰胆碱酯酶和α - 葡萄糖苷酶活性的抗氧化抑制作用。DPPH：1,1 - 二苯基 - 2 - 苦基肼，（）：，AChE：乙酰胆碱酯酶，BChE：丁酰胆碱酯酶，AD：阿尔茨海默病
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