Xu Zhili, Liang Hanye, Zhang Mingbo, Tao Xiaojun, Dou Deqiang, Hu Liping, Kang Tingguo
College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Liaoning 116600, PR China.
College of Information Science & Technology, Liaoning University of Traditional Chinese Medicine, Liaoning 110847, PR China.
Korean J Physiol Pharmacol. 2017 Nov;21(6):609-616. doi: 10.4196/kjpp.2017.21.6.609. Epub 2017 Oct 30.
Ardipusilloside-I is a natural triterpenoid saponin, which was isolated from A. DC. The aim of the study was to evaluate the stimulation of ardipusilloside-I on gastrointestinal motility and . The experiment of smooth muscle contraction directly monitored the contractions of the isolated jejunal segment (IJS) in different contractile states, and the effects of ardipusilloside-I on myosin were measured in the presence of Ca-calmodulin using the activities of 20 kDa myosin light chain (MLC) phosphorylation and myosin Mg-ATPase. The effects of ardipusilloside-I on gastro emptying and intestinal transit in constipation-predominant rats were observed, and the MLCK expression in jejuna of constipated rats was determined by western blot. The results showed that, ardipusilloside-I increased the contractility of IJS in a dose-dependent manner and reversed the low contractile state (LCS) of IJS induced by low Ca, adrenaline, and atropine respectively. There were synergistic effects on contractivity of IJS between ardipusilloside-I and ACh, high Ca, and histamine, respectively. Ardipusilloside-I could stimulate the phosphorylation of MLC and Mg-ATPase activities of Ca- dependent phosphorylated myosin. Ardipusilloside-I also stimulated the gastric emptying and intestinal transit in normal and constipated rats , respectively, and increased the MLCK expression in the jejuna of constipation-predominant rats. Briefly, the findings demonstrated that ardipusilloside-I could effectively excite gastrointestinal motility and .
地锦草皂苷-I是一种天然三萜皂苷，从地锦草中分离得到。本研究的目的是评估地锦草皂苷-I对胃肠动力的刺激作用。平滑肌收缩实验直接监测不同收缩状态下离体空肠段（IJS）的收缩情况，并在钙调蛋白存在的情况下，利用20 kDa肌球蛋白轻链（MLC）磷酸化活性和肌球蛋白Mg-ATP酶来测定地锦草皂苷-I对肌球蛋白的影响。观察地锦草皂苷-I对便秘型大鼠胃排空和肠道传输的影响，并通过蛋白质免疫印迹法测定便秘大鼠空肠中MLCK的表达。结果表明，地锦草皂苷-I以剂量依赖性方式增加IJS的收缩力，并分别逆转由低钙、肾上腺素和阿托品诱导的IJS低收缩状态。地锦草皂苷-I与乙酰胆碱、高钙和组胺之间分别对IJS的收缩性有协同作用。地锦草皂苷-I可刺激MLC的磷酸化以及钙依赖性磷酸化肌球蛋白的Mg-ATP酶活性。地锦草皂苷-I还分别刺激正常和便秘大鼠的胃排空和肠道传输，并增加便秘型大鼠空肠中MLCK的表达。简而言之，研究结果表明地锦草皂苷-I可有效激发胃肠动力。
