Zhang Yi, Tang Lili
Department of Breast Surgery, Xiangya Hospital Central South University, No. 87 Xiangya Road, Changsha, 410008 China.
Cancer Cell Int. 2017 Nov 21;17:109. doi: 10.1186/s12935-017-0480-0. eCollection 2017.
Recent studies showed that long non-coding RNA (lncRNA) plays an important role in many life activities. is one of the lncRNA genes that are expressed differently between breast cancer and normal tissues by the lncRNA gene chip. Our study was conducted to examine the regulation of lncRNA in breast cancer.
Two cell lines, MCF-7 and MDA-MB-231, were selected to be the research objects in this study; overexpression and knockdown models were established by transforming vectors. Real-time polymerase chain reaction, MTT assay, clone formation and cell flow cytometer assay were used to test the function of . Dual-luciferase assay was used to detect a target relationship between and miR-122.
overexpression promoted cell cycle and proliferation and increased colony formation. In the overexpression model, there was a target relationship between and miR-122, and some of the downstream genes of miR-122, including and , were increased. Moreover, we found that lentivirus-mediated interference of lncRNA inhibited tumour growth in nude mice.
Breast cancer progression can be promoted by directly targeting miR-122 through lncRNA . This study provided evidence that can serve as the molecular basis for improving treatment options for patients.
近期研究表明，长链非编码RNA（lncRNA）在许多生命活动中发挥重要作用。 是通过lncRNA基因芯片在乳腺癌组织和正常组织中表达存在差异的lncRNA基因之一。本研究旨在探讨lncRNA 在乳腺癌中的调控作用。
选取MCF-7和MDA-MB-231两种细胞系作为研究对象；通过转染载体建立过表达和敲低模型。采用实时聚合酶链反应、MTT法、克隆形成实验和细胞流式仪检测法检测 的功能。采用双荧光素酶报告基因检测法检测 与miR-122之间的靶向关系。
过表达促进细胞周期和增殖，并增加克隆形成。在过表达模型中， 与miR-122之间存在靶向关系，miR-122的一些下游基因，包括 和 ，表达增加。此外，我们发现慢病毒介导的lncRNA 干扰可抑制裸鼠肿瘤生长。
lncRNA 可通过直接靶向miR-122促进乳腺癌进展。本研究为改善患者治疗方案提供了分子基础依据。
