Inhibition of breast cancer cell proliferation and tumorigenesis by long non-coding RNA down-regulation of miR-122 expression.

BACKGROUND

Recent studies showed that long non-coding RNA (lncRNA) plays an important role in many life activities. is one of the lncRNA genes that are expressed differently between breast cancer and normal tissues by the lncRNA gene chip. Our study was conducted to examine the regulation of lncRNA in breast cancer.

METHODS

Two cell lines, MCF-7 and MDA-MB-231, were selected to be the research objects in this study; overexpression and knockdown models were established by transforming vectors. Real-time polymerase chain reaction, MTT assay, clone formation and cell flow cytometer assay were used to test the function of . Dual-luciferase assay was used to detect a target relationship between and miR-122.

RESULTS

overexpression promoted cell cycle and proliferation and increased colony formation. In the overexpression model, there was a target relationship between and miR-122, and some of the downstream genes of miR-122, including and , were increased. Moreover, we found that lentivirus-mediated interference of lncRNA inhibited tumour growth in nude mice.

CONCLUSION

Breast cancer progression can be promoted by directly targeting miR-122 through lncRNA . This study provided evidence that can serve as the molecular basis for improving treatment options for patients.