Moradi Mohammad, Mojab Faraz, Arbabi Bidgoli Sepideh
Pharmaceutical Sciences Research Center, Islamic Azad University (IAUPS), Pharmaceutical Sciences Branch, Tehran, Iran.
Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran.
Iran J Pharm Res. 2017 Summer;16(3):1071-1079.
The species Asteraceae Centaurea repens (Asteraceae), known as Acroptilon repens, and Talkhe in persian is used in folk medicine as an emetic, anti-epileptic, and anti-malaria herb in many parts of the world but its toxic effects have not determined yet. This study aimed to evaluate the acute and subchronic toxicity of this extract to find its possible adverse health effects through clinical, hematological, biochemical, and histopathological endpoints in both gender of mice. Aerial parts of the plant were air-dried and the terpene extract of aerial parts of plant was provided by percolation using methanol, petroleum ether, and diethyl ether. All clinical, biochemical and histopathological changes were assessed in appropriate endpoints and compared with control group. Although no mortality was seen in acute study by administrating doses up to 2000 mg/kg, repeated dose study on 1000 mg/kg doses in 28 days in both genders showed liver necrosis and rise of liver enzymes (p-value < 0.05). Histopathological studies didn't show any other organ toxicity in dosed up to 1000 mg/kg. At the same time this study showed for the first the antihyperlipidemic properties of the aerial extract of Acroptilin in mice model. The pharmacological and histopathological results of the present study proved that the total parts of could be studied for supporting the traditional assertion in folk medicine to heal hyperlipidemia, diabetes, and cancer in lower doses although we performed the present study and concluded liver toxicity by subchronic use of extract.
菊科植物顶羽菊（学名：Acroptilon repens，波斯语称为Talkhe），在世界许多地区的民间医学中被用作催吐剂、抗癫痫药和抗疟疾草药，但其毒性作用尚未确定。本研究旨在评估该提取物的急性和亚慢性毒性，通过小鼠雌雄两性的临床、血液学、生化和组织病理学指标来发现其可能对健康产生的不良影响。将植物地上部分风干，通过用甲醇、石油醚和乙醚渗漉法制备植物地上部分的萜类提取物。在适当的指标中评估所有临床、生化和组织病理学变化，并与对照组进行比较。虽然在急性研究中给予高达2000mg/kg的剂量未观察到死亡，但在28天内对两性给予1000mg/kg剂量的重复给药研究显示肝坏死和肝酶升高（p值<0.05）。组织病理学研究未显示在给予高达1000mg/kg剂量时其他器官有任何毒性。同时，本研究首次显示顶羽菊地上提取物在小鼠模型中具有抗高血脂特性。本研究的药理学和组织病理学结果证明，尽管我们进行了本研究并得出亚慢性使用该提取物会导致肝毒性的结论，但顶羽菊的所有部分仍可在较低剂量下进行研究，以支持民间医学中关于治疗高血脂、糖尿病和癌症的传统说法。
