Kamyab Mostafa, Kim Euikyung, Hoseiny Seyed Mehdi, Seyedian Ramin
Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.
Department of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju, South Korea.
Iran J Pharm Res. 2017 Summer;16(3):1155-1160.
Snakebite is a common problem especially in tropical areas all over the world including Iran. as one of the most dangerous Iranian snakes is spreading in this country excluding central and northwest provinces. In this study gelatinase and fibrinogenolytic properties as two disintegrating matrix metalloproteinase enzymes were evaluated by a strong clear halo between 56-72 kDa in addition to another band located 76-102 kDa for gelatinase and one major band around 38 kDa for fibrinogenolytic enzyme respectively. The electrophorectc profile of our venom demonstrated at least one protein band between 24-31 kDa like previous reports and another two bands between 52-76 kDa and below 17 kDa stemmed probably due to the effect of natural selection in one species. According to our results Razi institute antivenin could neutralize effects of gelatinase enzyme comprehensively. The electrophoretic profile of Iranian commercial antivenom as the main intravenous treatment of envenomed patients showed impurities in addition to F (ab') weighing 96 kDa in SDS-PAGE analysis. It proposes more efforts for refinement to avoid short and long unwanted effects in envenomed patients.
蛇咬伤是一个常见问题，尤其是在包括伊朗在内的世界热带地区。作为伊朗最危险的蛇类之一，它正在该国除中部和西北部省份以外的地区蔓延。在本研究中，对明胶酶和纤维蛋白原溶解特性这两种分解基质金属蛋白酶进行了评估，除了明胶酶在56 - 72 kDa之间有一条强清晰条带以及另一条位于76 - 102 kDa的条带外，纤维蛋白原溶解酶分别在约38 kDa处有一条主要条带。我们毒液的电泳图谱显示，如先前报道，在24 - 31 kDa之间至少有一条蛋白条带，另外在52 - 76 kDa和低于17 kDa之间有两条条带，这可能是由于一个物种的自然选择作用。根据我们的结果，拉齐研究所抗蛇毒血清可以全面中和明胶酶的作用。伊朗商业抗蛇毒血清作为中毒患者的主要静脉治疗药物，其电泳图谱在SDS - PAGE分析中除了96 kDa的F(ab')外还显示有杂质。这表明需要进一步努力进行提纯，以避免对中毒患者产生短期和长期的不良影响。
