Masjedi Mohsen, Esmaeil Nafiseh, Saffaei Ali, Abtahi-Naeini Bahareh, Pourazizi Mohsen, Haghjooy Javanmard Shaghayegh, Asilian Ali
Department of Immunology, Isfahan University of Medical Science, Isfahan, Iran.
Pharmacy Students' Research Committee, School of Pharmacy, Isfahan University of Medical sciences, Isfahan, Iran.
Iran J Pharm Res. 2017 Summer;16(3):1223-1229.
Pemphigus vulgaris (PV) is a chronic autoimmune blistering disease of the skin, in which loss of adhesion between keratinocytes is caused by autoantibodies. It has been hypothesized that cytokines play an essential role in the pathogenesis of PV. This study aimed to investigate the other immunopathological aspects of PV by determining the serum levels of cytokines in PV patients to find another treatment strategy except corticosteroid therapy. Twenty-three patients with PV and a control group consisting of 24 healthy subjects were studied. Interleukin (IL)-2, IL-4, IL-6, IL10, IL-12, IL-17 and interferon-gamma (IFN-γ) were measured in the sera of patients by the enzyme-linked immunosorbent assay (ELISA) method. The serum levels of IL-2, IL-4, IL-17 and IFN-γ in most patients and controls were undetectable. The serum concentrations of IL-10 in the patients and controls were undetectable, nevertheless, the mean serum levels of this cytokine was 64.375 pg/mL in four patients. The mean serum levels of pro-inflammatory cytokine IL-6 increased significantly in the patients, compared to the controls (169.50 vs. 75.62 pg/mL) (P < 0.05). The same was observed for another pro-inflammatory cytokine, IL-12 (135.33 vs. 86.28 pg/mL) (P < 0.05). Based on the results of this study it can be concluded that the Type 2 T helper cytokine (IL-6) and macrophage-derived cytokine (IL-12) have essential roles in PV pathophysiology. In addition, the potential clinical application of Th1/Th2 type cytokine-based therapy in PV should be considered in next studies.
寻常型天疱疮（PV）是一种慢性自身免疫性皮肤水疱病，其中角质形成细胞之间的黏附丧失是由自身抗体引起的。据推测，细胞因子在PV的发病机制中起重要作用。本研究旨在通过测定PV患者血清中细胞因子水平来研究PV的其他免疫病理方面，以寻找除皮质类固醇治疗之外的另一种治疗策略。研究了23例PV患者和由24名健康受试者组成的对照组。采用酶联免疫吸附测定（ELISA）法测定患者血清中的白细胞介素（IL）-2、IL-4、IL-6、IL10、IL-12、IL-17和干扰素-γ（IFN-γ）。大多数患者和对照组血清中的IL-2、IL-4、IL-17和IFN-γ水平无法检测到。患者和对照组血清中IL-10的浓度无法检测到，然而，4例患者中该细胞因子的平均血清水平为64.375 pg/mL。与对照组相比，患者中促炎细胞因子IL-6的平均血清水平显著升高（169.50对75.62 pg/mL）（P<0.05）。另一种促炎细胞因子IL-12也观察到同样情况（135.33对86.28 pg/mL）（P<0.05）。基于本研究结果可以得出结论，2型辅助性T细胞细胞因子（IL-6）和巨噬细胞衍生细胞因子（IL-12）在PV病理生理学中起重要作用。此外，后续研究应考虑Th1/Th2型细胞因子疗法在PV中的潜在临床应用。
