Ge Fei, Zhu Shilin, Liu Lina, Yan Jing, Ji Yu, Sun Zhiguang
Department of Gastroenterology, Haian Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Haian, Jiangsu 226600, P.R. China.
First College of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, P.R. China.
Exp Ther Med. 2017 Nov;14(5):4745-4754. doi: 10.3892/etm.2017.5153. Epub 2017 Sep 20.
(Trev.) Meisn (Fag), which belongs to the Polygonaceae family, has been widely used to treat inflammatory diseases. Previous studies have revealed that Fag components exhibit anti-inflammatory activities; however, their potential use in treating inflammatory bowel disease (IBD) has not been explored. In the present study, mice with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis were used as a model of IBD. Fag extract was orally administered for 3 days following the induction of colitis and the conventional drug, salicylazosulfapyridine (SASP), was used as a control. The results revealed that Fag significantly ameliorated TNBS-induced body weight loss and colonic shortening in mice (P<0.05). Furthermore, Fag suppressed levels of proinflammatory cytokines and reduced macrophage infiltration into colonic tissues (P<0.05). To further verify the anti-inflammatory effects of Fag at the molecular level, a murine macrophage cell line, Raw264.7, was used. Nuclear translocation of nuclear factor (NF)-κB p65 and the phosphorylation of inhibitor of NF-κB (IκB) were assessed using western blotting. The results demonstrated that Fag inhibited the production of proinflammatory cytokines via inhibiting NF-κB p65 nuclear translocation and IκB phosphorylation (P<0.05). Furthermore, the clinical study results revealed that Fag had significantly fewer side effects (P<0.05) and served as a better anti-inflammatory drug for ulcerative colitis compared with SASP.
（Trev.）Meisn（蓼属）属于蓼科，已被广泛用于治疗炎症性疾病。先前的研究表明，蓼属成分具有抗炎活性；然而，它们在治疗炎症性肠病（IBD）方面的潜在用途尚未得到探索。在本研究中，将2,4,6-三硝基苯磺酸（TNBS）诱导的结肠炎小鼠用作IBD模型。在诱导结肠炎后口服蓼属提取物3天，并使用传统药物柳氮磺胺吡啶（SASP）作为对照。结果显示，蓼属显著改善了TNBS诱导的小鼠体重减轻和结肠缩短（P<0.05）。此外，蓼属抑制促炎细胞因子水平并减少巨噬细胞向结肠组织的浸润（P<0.05）。为了在分子水平上进一步验证蓼属的抗炎作用，使用了小鼠巨噬细胞系Raw264.7。使用蛋白质印迹法评估核因子（NF）-κB p65的核转位和NF-κB抑制剂（IκB）的磷酸化。结果表明，蓼属通过抑制NF-κB p65核转位和IκB磷酸化来抑制促炎细胞因子的产生（P<0.05）。此外，临床研究结果显示，与SASP相比，蓼属的副作用明显更少（P<0.05），是治疗溃疡性结肠炎更好的抗炎药物。
