Department of Blood Transfusion Huashan Hospital Fudan University Shanghai China.
Medical Faculty Kunming University of Science and Technology Kunming China.
Brain Behav. 2017 Oct 18;7(11):e00854. doi: 10.1002/brb3.854. eCollection 2017 Nov.
Stroke may cause neuropsychiatric problems, which have negative effects on cognitive functions and behavior. Exercise plays an important role in reducing the occurrence and development of stroke, the concrete mechanism is not fully clarified. In this study, we attempted to determine whether early treadmill exercise attenuates anxiety-like behavior by regulation of inflammation after brain ischemia.
We subjected adult male rats to middle cerebral artery occlusion (MCAO) for 90 min and trained rats started to run on a treadmill from postoperative day 1 to day 14. The effects of treadmill on cognitive functions, anxiety-like behavior, and immune activation were analyzed by Morris water maze test, open field test, elevated plus maze test, and enzyme-linked immunosorbent assay.
Early treadmill exercise significantly improved cognitive function, alleviated anxiety-like behavior in ischemic rats model; this improvement was associated with significantly decreased activation of astrocytes and microglia cells and proinflammatory markers (platelet-activating factor [PAF], interleukin-6 [IL-6], tumor necrosis factor-alpha [TNF-α], intercellular adhesion molecule-1 [ICAM-1], and vascular cell adhesion molecule-1 [VCAM-1]).
Our results indicated that early treadmill exercise attenuated anxiety-like behavior by decreasing inflammation response, exercise conferred a great benefit of attenuating anxiety-like behavior via anti-inflammatory treatment may prove to be a novel neuroprotective strategy for stroke.
中风可能导致神经精神问题，对认知功能和行为产生负面影响。运动在减少中风的发生和发展方面起着重要作用，但其具体机制尚不完全清楚。在这项研究中，我们试图确定早期跑步机运动是否通过调节脑缺血后的炎症反应来减轻焦虑样行为。
我们使成年雄性大鼠经历 90 分钟的大脑中动脉闭塞（MCAO），并使大鼠从术后第 1 天开始到第 14 天开始在跑步机上跑步。通过 Morris 水迷宫测试、旷场测试、高架十字迷宫测试和酶联免疫吸附测定分析跑步机对认知功能、焦虑样行为和免疫激活的影响。
早期跑步机运动显著改善了缺血性大鼠模型的认知功能，减轻了焦虑样行为；这种改善与星形胶质细胞和小胶质细胞以及促炎标志物（血小板激活因子[PAF]、白细胞介素-6[IL-6]、肿瘤坏死因子-α[TNF-α]、细胞间黏附分子-1[ICAM-1]和血管细胞黏附分子-1[VCAM-1]）的激活显著降低有关。
我们的结果表明，早期跑步机运动通过降低炎症反应来减轻焦虑样行为，运动通过抗炎治疗来减轻焦虑样行为可能是中风的一种新的神经保护策略。
