慢性乙型肝炎肝细胞中的乙肝核心抗原：间接免疫荧光法与免疫过氧化物酶法的比较

Hepatitis B Core Antigen in Hepatocytes of Chronic Hepatitis B: Comparison between Indirect Immunofluorescence and Immunoperoxidase Method.

作者信息

Raihan Ruksana, Tabassum Shahina, Al-Mahtab Mamun, Nessa Afzalun, Jahan Munira, Shamim Kabir Chowdhury Mohammad, Kamal Mohammad, Cesar Aguilar Julio

机构信息

Department of Microbiology, Faculty of Medicine, AIMST University, Bedong, Kedah, Malaysia.

Department of Virology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka, Bangladesh.

出版信息

Euroasian J Hepatogastroenterol. 2015 Jan-Jun;5(1):7-10. doi: 10.5005/jp-journals-10018-1120. Epub 2015 Jan 6.

DOI:10.5005/jp-journals-10018-1120

PMID:29201677

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5578511/

Abstract

BACKGROUND

Hepatitis B virus (HBV) infection has many faces. Precore and core promoter mutants resemble inactive carrier status. The identification of hepatitis B core antigen (HBcAg) in hepatocytes may have variable clinical significance. The present study was undertaken to detect HBcAg in chronic hepatitis B (CHB) patients and to assess the efficacy of detection system by indirect immunofluorescence (IIF) and indirect immunoperoxidase (IIP).

MATERIALS AND METHODS

The study was done in 70 chronic HBV-infected patients. Out of 70 patients, eight (11.4%) were hepatitis B e antigen (HBeAg) positive and 62 (88.57%) were HBeAg negative. Hepatitis B core antigen was detected by indirect immunofluorescence (IIF) and indirect immunoperoxidase (IIP) methods in liver tissue.

RESULTS

All HBeAg positive patients expressed HBcAg by both IIF and IIP methods. Out of 62 patients with HBeAg-negative CHB, HBcAg was detected by IIF in 55 (88.7%) patients and by IIP in 51 (82.26%) patients. A positive relation among viral load and HBcAg detection was also found. This was more evident in the case of HBeAg negative patients and showed a positive relation with HBV DNA levels.

CONCLUSION

Hepatitis B core antigen can be detected using the IIF from formalin fixed paraffin block preparation and also by IIP method. This seems to reflect the magnitudes of HBV replication in CHB.

HOW TO CITE THIS ARTICLE

Raihan R, Tabassum S, Al-Mahtab M, Nessa A, Jahan M, Kabir CMS, Kamal M, Aguilar JC. Hepatitis B Core Antigen in Hepatocytes of Chronic Hepatitis B: Comparison between Indirect Immunofluorescence and Immunoperoxidase Method. Euroasian J Hepato-Gastroenterol 2015;5(1):7-10.

摘要

背景

乙型肝炎病毒（HBV）感染具有多种表现形式。前核心和核心启动子突变类似于非活动性携带者状态。肝细胞中乙型肝炎核心抗原（HBcAg）的鉴定可能具有不同的临床意义。本研究旨在检测慢性乙型肝炎（CHB）患者中的HBcAg，并通过间接免疫荧光法（IIF）和间接免疫过氧化物酶法（IIP）评估检测系统的有效性。

材料与方法

本研究纳入了70例慢性HBV感染患者。在这70例患者中，8例（11.4%）乙型肝炎e抗原（HBeAg）阳性，62例（88.57%）HBeAg阴性。采用间接免疫荧光法（IIF）和间接免疫过氧化物酶法（IIP）在肝组织中检测乙型肝炎核心抗原。

结果

所有HBeAg阳性患者通过IIF和IIP方法均表达HBcAg。在62例HBeAg阴性的CHB患者中，通过IIF检测到55例（88.7%）患者有HBcAg，通过IIP检测到51例（82.26%）患者有HBcAg。还发现病毒载量与HBcAg检测之间存在正相关。在HBeAg阴性患者中这种相关性更明显，且与HBV DNA水平呈正相关。

结论

可以使用福尔马林固定石蜡块制备的IIF以及IIP方法检测乙型肝炎核心抗原。这似乎反映了CHB中HBV复制的程度。

如何引用本文

Raihan R, Tabassum S, Al-Mahtab M, Nessa A, Jahan M, Kabir CMS, Kamal M, Aguilar JC. 慢性乙型肝炎肝细胞中的乙型肝炎核心抗原：间接免疫荧光法和免疫过氧化物酶法的比较。《欧亚肝脏胃肠病学杂志》2015；5(1):7 - 10。