Shandong University of Traditional Chinese Medicine, 4655 Daxue Road, Changqing District, Jinan, Shandong Province, China.
Affiliated Hospital of Shandong University of Traditional Chinese Medicine, 16369 Jingshi Road, Lixia District, Jinan, Shandong Province, China.
Biomed Res Int. 2017;2017:6761549. doi: 10.1155/2017/6761549. Epub 2017 Oct 22.
Essential hypertension (EH) is a risk factor for some severe diseases. This study aimed to screen out serum special proteins and seek interaction between them, which would provide new therapeutic targets and elucidate the comprehensive pathophysiological mechanism for EH. Patients with EH (Group A, = 47) and healthy controls (HC) (Group B, = 47) were recruited in this study. Serums from the two groups were analyzed with isobaric tags for relative and absolute quantitation coupled two-dimensional liquid chromatography followed by electrospray ionization-tandem mass spectrometry technique, while the candidate special proteins were verified with ELISA and western blot. A total of 404 proteins were identified, of which 30 proteins were upregulated (>1.2-fold, < 0.05) and 81 proteins were downregulated (<0.833-fold, < 0.05) compared with HC group. With GO, KEGG analysis, and literature retrieval, 4 proteins, cathepsin G, transforming growth factor beta-1, hyaluronidase-1, and kininogen-1, were found jointly involved in the renin-angiotensin-aldosterone system and kallikrein-kinin system. The profiles of these 4 candidate proteins were confirmed with ELISA and western blot. The concentration variation of these 4 proteins could better predict the occurrence and illustrate the pathophysiological mechanism of EH. And their discovery may help pave the way for exploring new therapies of EH.
原发性高血压（EH）是某些严重疾病的危险因素。本研究旨在筛选血清特殊蛋白并寻找它们之间的相互作用，为 EH 提供新的治疗靶点，并阐明其全面的病理生理机制。本研究纳入了 47 例 EH 患者（A 组）和 47 例健康对照者（B 组）。用相对和绝对定量同位素标记（iTRAQ）联合二维液相色谱-电喷雾串联质谱技术分析两组血清，用 ELISA 和 Western blot 验证候选特殊蛋白。共鉴定出 404 种蛋白，其中 30 种蛋白上调（>1.2 倍，<0.05），81 种蛋白下调（<0.833 倍，<0.05）。通过 GO、KEGG 分析和文献检索，发现 4 种蛋白（组织蛋白酶 G、转化生长因子-β1、透明质酸酶-1 和激肽原-1）共同参与了肾素-血管紧张素-醛固酮系统和激肽释放酶-激肽系统。这 4 种候选蛋白的图谱通过 ELISA 和 Western blot 得到了证实。这 4 种蛋白的浓度变化可以更好地预测 EH 的发生并阐明其病理生理机制。它们的发现可能有助于为 EH 的新疗法探索铺平道路。
