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应用等压标签相对和绝对定量技术（iTRAQ）联合二维液相色谱/串联质谱在特发性肺纤维化血清生物标志物发现中的定量蛋白质组学分析。

Application of Isobaric Tags for Relative and Absolute Quantification (iTRAQ) Coupled with Two-Dimensional Liquid Chromatography/Tandem Mass Spectrometry in Quantitative Proteomic Analysis for Discovery of Serum Biomarkers for Idiopathic Pulmonary Fibrosis.

机构信息

Department of Respiratory Medicine, The Second Hospital of Shandong University, Jinan, Shandong, China (mainland).

Central Laboratory, The Second Hospital of Shandong University, Jinan, Shandong, China (mainland).

出版信息

Med Sci Monit. 2018 Jun 17;24:4146-4153. doi: 10.12659/MSM.908702.

DOI:10.12659/MSM.908702
PMID:29909421
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6036962/
Abstract

BACKGROUND The present study was performed to explore the presence of informative protein biomarkers of human serum proteome in idiopathic pulmonary fibrosis (IPF). MATERIAL AND METHODS Serum samples were profiled using iTRAQ coupled with two-dimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS) technique, and ELISA was used to validate candidate biomarkers. RESULTS A total of 394 proteins were identified and 97 proteins were associated with IPF. Four biomarker candidates generated from iTRAQ experiments - CRP, fibrinogen-α chain, haptoglobin, and kininogen-1 - were successfully verified using ELISA. CONCLUSIONS The present study demonstrates that levels of CRP and fibrinogen-a are higher and levels of haptoglobin and kininogen-1 are lower in patients with IPF compared to levels in healthy controls. We found they are useful candidate biomarkers for IPF.

摘要

背景

本研究旨在探索特发性肺纤维化（IPF）患者血清蛋白质组中是否存在信息丰富的蛋白质生物标志物。

材料与方法

采用 iTRAQ 联合二维液相色谱/串联质谱（2D-LC-MS/MS）技术对血清样本进行分析，并采用 ELISA 法对候选生物标志物进行验证。

结果

共鉴定出 394 种蛋白质，其中 97 种与 IPF 相关。从 iTRAQ 实验中生成的 4 种生物标志物候选物 - CRP、纤维蛋白原-α 链、触珠蛋白和激肽原-1 - 成功地通过 ELISA 验证。

结论

本研究表明，与健康对照组相比，IPF 患者的 CRP 和纤维蛋白原-α 水平较高，而触珠蛋白和激肽原-1 水平较低。我们发现它们是 IPF 的有用候选生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b825/6036962/303684bf347a/medscimonit-24-4146-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b825/6036962/536c9631a7f2/medscimonit-24-4146-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b825/6036962/303684bf347a/medscimonit-24-4146-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b825/6036962/536c9631a7f2/medscimonit-24-4146-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b825/6036962/bd10eca01c49/medscimonit-24-4146-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b825/6036962/0a8f4e6c08a7/medscimonit-24-4146-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b825/6036962/303684bf347a/medscimonit-24-4146-g004.jpg

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