Daly Matthew, Ansari Parisa, Häubl Georg, Rogers Adrian, Brunner Kurt
Romer Laboratories UK Ltd, The Heath Business and Technical Park, Runcorn, Cheshire, United Kingdom.
Romer Laboratories Division Holding GmbH, Technopark 1, 3430 Tulln, Austria.
J AOAC Int. 2018 Jan 1;101(1):96-101. doi: 10.5740/jaoacint.17-0398. Epub 2017 Dec 5.
With an ever-increasing allergic population and an emerging market for allergen-free foods, accurate detection of allergens in foods has never been more important. Although ELISA-based methods are the most widely used for detection of allergens in food, there is a need for the development of orthogonal approaches. A commercial ELISA detected a relatively high concentration of peanut and almond in an allergen-free product. However, another commercial ELISA declared a low peanut concentration and was negative for almond. Further testing using a commercial almond lateral-flow device confirmed the results from the second ELISA kit and demonstrated that the positive detection of almond was due to cross-reactivity. An MS method was used for final confirmation that the reported results were negative for both almond and peanut.
随着过敏人群的不断增加以及无过敏原食品市场的兴起，准确检测食品中的过敏原变得前所未有的重要。尽管基于酶联免疫吸附测定（ELISA）的方法是食品中过敏原检测最广泛使用的方法，但仍需要开发正交方法。一种商业ELISA检测到一种无过敏原产品中花生和杏仁的浓度相对较高。然而，另一种商业ELISA检测出花生浓度较低且杏仁呈阴性。使用商用杏仁侧向流动装置进行的进一步测试证实了第二种ELISA试剂盒的结果，并表明杏仁的阳性检测是由于交叉反应。采用质谱（MS）方法进行最终确认，结果表明所报告的产品中杏仁和花生均呈阴性。
